The ultrasound contrast imaging properties of lipid microbubbles loaded with urokinase in dog livers and their thrombolytic effects when combined with low-frequency ultrasound in vitro
| العنوان: | The ultrasound contrast imaging properties of lipid microbubbles loaded with urokinase in dog livers and their thrombolytic effects when combined with low-frequency ultrasound in vitro |
|---|---|
| المؤلفون: | Xiao-Ning Kang, Li-Fang Tan, Xin-Liang Shen, Rong-Guo Fu, Shu-Ting Ren, Hui-Ting Luo, Yi-Ran Liao, Bing Wang, Wang Wei, Lina Chen, Hong Ai |
| المصدر: | Journal of Thrombosis and Thrombolysis. 37:303-309 |
| بيانات النشر: | Springer Science and Business Media LLC, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | medicine.medical_specialty, animal structures, medicine.medical_treatment, Contrast Media, Contrast imaging, Dogs, Fibrinolytic Agents, medicine, Zeta potential, Animals, Thrombolytic Therapy, Ultrasonography, Urokinase, Microbubbles, business.industry, Ultrasound, Echogenicity, Thrombosis, Hematology, Thrombolysis, Urokinase-Type Plasminogen Activator, In vitro, Liver, Radiology, Cardiology and Cardiovascular Medicine, business, medicine.drug, Biomedical engineering |
| الوصف: | A new microbubble loaded with urokinase (uPA-MB) was explored in a previous study. However, its zeta potential and ultrasound contrast imaging properties and its thrombolytic effects when combined with low-frequency ultrasound (LFUS) were unclear. The zeta potential and ultrasound contrast imaging property of 5 uPA-MBs loading with 50,000 IU uPA was respectively detected using a Malvern laser particle analyzer and a Logiq 9 digital premium ultrasound system. Its ultrasound contrast imaging property was performed on the livers of two healthy dogs to compare with SonoVue. And the clot mass loss rate, D-dimer concentration and surface morphology of the clot residues were measured to evaluate the thrombolytic effect after treatment with three doses of 5 uPA-MBs combined with LFUS in vitro. The zeta potential of 5 uPA-MBs (-27.0 ± 2.40 mV) was higher than that of normal microbubbles (-36.95 ± 1.77 mV). Contrast-enhanced imaging of the hepatic vessels using 5 uPA-MBs was similar to SonoVue, while the imaging duration of 5 uPA-MBs (10 min) was longer than SonoVue (6 min). The thrombolytic effect of three doses of uPA-MBs combined with LFUS was significantly better than that of the control group and showed dose dependence. The 5 uPA-MBs have a negative charge on their surface and good echogenicity as ultrasound contrast agents. The 5 uPA-MBs combined with LFUS can promote thrombolysis in a dose-dependent manner. |
| تدمد: | 1573-742X 0929-5305 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2ff31bea75a81b585aefc71328613e21 https://doi.org/10.1007/s11239-013-0950-8 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....2ff31bea75a81b585aefc71328613e21 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 1573742X 09295305 |
|---|