SD118-xanthocillin X (1), a novel marine agent extracted from Penicillium commune, induces autophagy through the inhibition of the MEK/ERK pathway
| العنوان: | SD118-xanthocillin X (1), a novel marine agent extracted from Penicillium commune, induces autophagy through the inhibition of the MEK/ERK pathway |
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| المؤلفون: | Weizhang Sun, Bin Yuan, Ying Zhao, Bin-Gui Wang, Mingyong Miao, Binghua Jiao, Zhuo Shang, Caiguo Huang, Huan Chen |
| المصدر: | Marine Drugs Marine Drugs; Volume 10; Issue 6; Pages: 1345-1359 |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | MAPK/ERK pathway, Aquatic Organisms, autophagy, MAP Kinase Signaling System, SD118-xanthocillin X (1), apoptosis, ERK, Beclin 1, Pharmaceutical Science, Down-Regulation, Biology, Article, chemistry.chemical_compound, Phosphatidylinositol 3-Kinases, Phenols, Cell Line, Tumor, Drug Discovery, Butadienes, Humans, SD118-xanthocillin X (1), Pharmacology, Toxicology and Pharmaceutics (miscellaneous), PI3K/AKT/mTOR pathway, Penicillium commune, Biological Products, Adenine, Autophagy, Penicillium, Membrane Proteins, Hep G2 Cells, Molecular biology, In vitro, Cell biology, Up-Regulation, chemistry, Apoptosis, Beclin-1, Growth inhibition, Signal transduction, Apoptosis Regulatory Proteins, Signal Transduction |
| الوصف: | A compound named SD118-xanthocillin X (1) (C(18)H(12)N(2)O(2)), isolated from Penicillium commune in a deep-sea sediment sample, has been shown to inhibit the growth of several cancer cell lines in vitro. In the present study, we employed a growth inhibition assay and apoptotic analysis to identify the biological effect and detailed mechanism of SD118-xanthocillin X (1) in human hepatocellular carcinoma (HepG2) cells. SD118-xanthocillin X (1) demonstrated a concentration-dependent inhibitory effect on the growth of HepG2 cells and caused slight cellular apoptosis and significantly induced autophagy. Autophagy was detected as early as 12 h by the conversion of microtubule-associated protein 1 light chain 3 (LC3-I) to LC3-II, following cleavage and lipid addition to LC3-I. The pharmacological autophagy inhibitor 3-methyladenine largely attenuates the growth inhibition and autophagic effect of SD118-xanthocillin X (1) in HepG2 cells. Our data also indicated that the autophagic effect of SD118-xanthocillin X (1) occurs via the down-regulation of the MEK/ERK signaling pathway and the up-regulated class III PI3K/Beclin 1 signaling pathway. |
| وصف الملف: | application/pdf |
| تدمد: | 1660-3397 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30819a9ecc89b66f6f7cc2954e90e4c2 https://pubmed.ncbi.nlm.nih.gov/22822377 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....30819a9ecc89b66f6f7cc2954e90e4c2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16603397 |
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