Catalyst-free site-selective cross-aldol bioconjugations
| العنوان: | Catalyst-free site-selective cross-aldol bioconjugations |
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| المؤلفون: | Nicholas D. J. Yates, Saeed Akkad, Amanda Noble, Tessa Keenan, Natasha E. Hatton, Nathalie Signoret, Martin A. Fascione |
| المصدر: | Green Chemistry. 24:8046-8053 |
| بيانات النشر: | Royal Society of Chemistry (RSC), 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Environmental Chemistry, Pollution |
| الوصف: | The bioconjugation of proteins to small molecules serves as an invaluable tool for probing biological mechanisms and creating biomaterials. The most powerful bioconjugation stratagems are those which have rapid kinetics, are site-selective, can be conducted in aqueous solvent under mild conditions and do not require the use of additional potentially toxic catalysts. Herein we present spontaneous coupling via aldol ligation of proteins (SCALP) a catalyst-free “green” site-selective protein ligation between α-oxo aldehyde functionalised proteins and enolisable aldehyde probes at neutral pH, and demonstrate the utility of this system in the targeting of prostate cancer cells with functionalised nanobodies. |
| وصف الملف: | application/pdf |
| تدمد: | 1463-9270 1463-9262 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::309d1583435604f4b4d6ab50d64f89a7 https://doi.org/10.1039/d2gc02292c |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....309d1583435604f4b4d6ab50d64f89a7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14639270 14639262 |
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