Study of the role of IL-17F gene polymorphism in the development of immune thrombocytopenia among the Egyptian children
| العنوان: | Study of the role of IL-17F gene polymorphism in the development of immune thrombocytopenia among the Egyptian children |
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| المؤلفون: | Alaa Gad, Ola M. Ibrahim, Shahira K. A. Botros |
| المصدر: | Egyptian Journal of Medical Human Genetics, Vol 19, Iss 4, Pp 385-389 (2018) Egyptian Journal of Medical Human Genetics; Vol 19, No 4 (2018); 385-389 |
| بيانات النشر: | SpringerOpen, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, lcsh:R5-920, lcsh:QH426-470, medicine.medical_treatment, Single-nucleotide polymorphism, Biology, 03 medical and health sciences, lcsh:Genetics, 030104 developmental biology, 0302 clinical medicine, Immune system, Cytokine, 030220 oncology & carcinogenesis, Immunology, Genotype, medicine, SNP, Gene polymorphism, Allele, lcsh:Medicine (General), Allele frequency, Genetics (clinical), Interleukin 17F (IL-17F), Primary immune thrombocytopenia (PIT), Single nucleotide polymorphism (SNP), Polymerase chain reaction-restriction, fragment polymorphism (PCR-RFLP) |
| الوصف: | Background: Interleukin 17F (IL-17F) is a pro-inflammatory cytokine that is recently proved to have a crucial role in the emergence of autoimmune diseases; it induces the expression of various cytokines, chemokines, and adhesion molecules. IL-17F polymorphism is subsequently related to enhanced IL-17F expression and activity; which may result in susceptibility to many autoimmune diseases including primary immune thrombocytopenia (PIT). Aim of the study: This case-control study aimed to investigate the possible association between IL and 17F gene single nucleotide polymorphism (SNP) at rs 7488A/G and PIT susceptibility in Egyptian pediatric patients. Subjects and methods: A total of 50 children with PIT with a mean age of 7 years, together with 50 age and sexmatched healthy controls were enrolled in the study for evaluation. Polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP) was used for detection of IL-17F polymorphism at rs7488A/G. Results: Regarding the genotypes distribution, the frequencies of the AA, AG and GG genotypes were 96, 2, and 2% in PIT patients and 90, 10 and 0% in the control group respectively. The A and G allele frequencies were 97 and 3% in the patients’ group versus 95 and 5% in the control group. There was no significant difference in either genotypes or allelic distribution between PIT patients and the controls. Conclusion: Our study suggests that IL17F gene polymorphism at rs7488A/G may not contribute to the susceptibility in development of primary immune thrombocytopenia in the Egyptian children. Keywords: Interleukin 17F (IL-17F), Primary immune thrombocytopenia (PIT), Single nucleotide polymorphism (SNP), Polymerase chain reaction-restriction, fragment polymorphism (PCR-RFLP) |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1110-8630 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::309dd3cd31ddfb2232e44b7c01235f84 http://www.sciencedirect.com/science/article/pii/S1110863018300235 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....309dd3cd31ddfb2232e44b7c01235f84 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 11108630 |
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