Relationship of leptin administration with production of reactive oxygen species, sperm DNA fragmentation, sperm parameters and hormone profile in the adult rat
| العنوان: | Relationship of leptin administration with production of reactive oxygen species, sperm DNA fragmentation, sperm parameters and hormone profile in the adult rat |
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| المؤلفون: | Azam Kouhkan, Abolfazl Kheimeh, Amir Akhlaghi, Abdolhossein Shahverdi, Shima Abbasihormozi, Javad Cheraghi |
| المصدر: | Archives of Gynecology and Obstetrics. 287:1241-1249 |
| بيانات النشر: | Springer Science and Business Media LLC, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Leptin, Male, medicine.medical_specialty, Adipose tissue, DNA Fragmentation, Biology, Semen analysis, Male infertility, Rats, Sprague-Dawley, Semen, Internal medicine, In Situ Nick-End Labeling, medicine, Animals, Testosterone, Sperm motility, medicine.diagnostic_test, Obstetrics and Gynecology, General Medicine, Luteinizing Hormone, medicine.disease, Spermatozoa, Sperm, Hormones, Rats, Fertility, Endocrinology, Sperm Motility, DNA fragmentation, Follicle Stimulating Hormone, Reactive Oxygen Species, Injections, Intraperitoneal |
| الوصف: | Leptin, an adipose tissue-derived hormone, plays an important role in energy homeostasis and metabolism, and in the neuroendocrine and reproductive systems. The function of leptin in male reproduction is unclear; however, it is known to affect sex hormones, sperm motility and its parameters. Leptin induces mitochondrial superoxide production in aortic endothelia and may increase oxidative stress and abnormal sperm production in leptin-treated rats. This study aims to evaluate whether exogenous leptin affects sperm parameters, hormone profiles, and the production of reactive oxygen species (ROS) in adult rats. A total of 65 Sprague–Dawley rats were divided into three treated groups and a control group. Treated rats received daily intraperitoneal injections of 5, 10 and 30 μg/kg of leptin administered for a duration of 7, 15, and 42 days. Control rats were given 0.1 mL of 0.9 % normal saline for the same period. One day after final drug administration, we evaluated serum specimens for follicle-stimulating hormone (FSH), leutinizing hormone (LH), free testosterone (FT), and total testosterone (TT) levels. Samples from the rat epididymis were also evaluated for sperm parameters and motility characteristics by a Computer-Aided Semen Analysis (CASA) system. Samples were treated with 2′,7′-dichlorofluorescein-diacetate (DCFH-DA) and analyzed using flow cytometry and TUNEL to determine the impact of leptin administration on sperm DNA fragmentation. According to CASA, significant differences in all sperm parameters in leptin-treated rats and their age-matched controls were detected, except for TM, ALH and BCF. Serum FSH and LH levels were significantly higher in rats that received 10 and 30 μg/kg of leptin compared to those treated with 5 μg/kg of leptin in the same group and control rats (P |
| تدمد: | 1432-0711 0932-0067 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30c98c5a2b1603dc9f56f254ed6ab722 https://doi.org/10.1007/s00404-012-2675-x |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....30c98c5a2b1603dc9f56f254ed6ab722 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14320711 09320067 |
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