Fibronectin/thermo-responsive polymer scaffold as a dynamic ex vivo niche for mesenchymal stem cells
| العنوان: | Fibronectin/thermo-responsive polymer scaffold as a dynamic ex vivo niche for mesenchymal stem cells |
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| المؤلفون: | Dencho Gugutkov, Roberto Guarino, Salima Nedjari, Laura Ramalho, Firas Awaja, George Altankov |
| المصدر: | Journal of Materials Science: Materials in Medicine. 31 |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Materials science, Polymers, 0206 medical engineering, Acrylic Resins, Biomedical Engineering, Biophysics, Adipose tissue, Bioengineering, 02 engineering and technology, In Vitro Techniques, Matrix (biology), Biomaterials, Tissue engineering, Osteogenesis, Materials Testing, Cell Adhesion, Humans, Cell Lineage, Cells, Cultured, Cellular Senescence, Cell Proliferation, Tissue Engineering, Tissue Scaffolds, biology, Stem Cells, Mesenchymal stem cell, Temperature, Cell Differentiation, Mesenchymal Stem Cells, Alkaline Phosphatase, 021001 nanoscience & nanotechnology, 020601 biomedical engineering, Culture Media, Fibronectins, Cell biology, Fibronectin, Adipose Tissue, biology.protein, Alkaline phosphatase, Calcium, Stem cell, 0210 nano-technology, Ex vivo |
| الوصف: | In this paper, we created a dynamic adhesive environment (DAE) for adipose tissue-derived mesenchymal stem cells (ADMSCs) cultured on smart thermo-responsive substrates, i.e., poly (N-isopropyl acrylamide) (PNIPAM), via introducing periodic changes in the culture temperature. We further explored the particular role of adsorbed fibronectin (FN), an important cell adhesive protein that was recently attributed to the recruitment of stem cells in the niche. The engineered FN/PNIPAM DAE system significantly increased the symmetric renewal of ADMSCs, particularly between passages 7 and 9 (p7-p9), before it dropped down to the level of the control (FN-coated TC polystyrene). This decline in the growth curve was consistent with the increased number of senescent cells, the augmented average cell size and the suppressed FN matrix secretion at late passages (p10-p12), all of them characteristic for stem cells ageing, which equivocally tended to slow down at our DAE system. FN supported also the osteogenic response of ADMSCs (apart from the previous observations with plain PNIPAM substrata) indicated by the significant increase of alkaline phosphatase (ALP) activity at days 7 and 14. The minimal changes in the Ca deposition, however, suggest a restricted effect of DAE on the early osteogenic response of ADMSCs only. Thus, the engineering of niche-like DAE involving FN uncovers a new tissue engineering strategy for gaining larger amounts of functionally active stem cells for clinical application. |
| تدمد: | 1573-4838 0957-4530 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30ef44122e9754cc51f2a7f0e991c5ef https://doi.org/10.1007/s10856-020-06461-y |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....30ef44122e9754cc51f2a7f0e991c5ef |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15734838 09574530 |
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