The CD34-like protein PODXL and α6-integrin (CD49f) identify early progenitor MSCs with increased clonogenicity and migration to infarcted heart in mice
| العنوان: | The CD34-like protein PODXL and α6-integrin (CD49f) identify early progenitor MSCs with increased clonogenicity and migration to infarcted heart in mice |
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| المؤلفون: | Min Jeong Seo, Andrey A. Pulin, Carl A. Gregory, Ryang Hwa Lee, Joni Ylostalo, Darwin J. Prockop |
| المصدر: | Blood. 113:816-826 |
| بيانات النشر: | American Society of Hematology, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Time Factors, Stromal cell, Hematopoiesis and Stem Cells, Sialoglycoproteins, Immunology, Myocardial Infarction, CD34, Antigens, CD34, Integrin alpha6, Biology, Kidney, Biochemistry, CXCR4, Epitopes, Mice, Cell Movement, medicine, Animals, Humans, Progenitor cell, Cells, Cultured, Oligonucleotide Array Sequence Analysis, Progenitor, Mesenchymal stem cell, Kidney metabolism, Mesenchymal Stem Cells, Cell Biology, Hematology, Molecular biology, medicine.anatomical_structure, Gene Expression Regulation, Cancer research, RNA Interference, Bone marrow |
| الوصف: | We screened for surface proteins expressed only by the early progenitor cells present in low-passage, low-density cultures of the adult stem/progenitor cells from bone marrow referred to as mesenchymal stem cells or multipotent stromal cells (MSCs). Six proteins were identified that were selectively expressed in the early progenitors: podocalyxin-like protein (PODXL), α6-integrin (CD49f), α4-integrin (CD49d), c-Met, CXCR4, and CX3CR1. All were previously shown to be involved in cell trafficking or tumor progression. Antibodies to CD49f and PODXL, a sialomucin in the CD34 family, were the most robust for FACScan assays. PODXLhi/CD49fhi MSCs were more clonogenic and differentiated more efficiently than PODXLlo/CD49flo cells. Inhibition of expression of PODXL with RNA interference caused aggregation of the cells. Furthermore, PODXLhi/CD49fhi MSCs were less prone to produce lethal pulmonary emboli, and larger numbers were recovered in heart and kidney after intravenous infusion into mice with myocardial infarcts. |
| تدمد: | 1528-0020 0006-4971 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30f95074e29e3f3da886a05af4e82088 https://doi.org/10.1182/blood-2007-12-128702 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....30f95074e29e3f3da886a05af4e82088 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15280020 00064971 |
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