Novel alternative PBX3 isoforms in leukemia cells with distinct interaction specificities
| العنوان: | Novel alternative PBX3 isoforms in leukemia cells with distinct interaction specificities |
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| المؤلفون: | Paul M. Watt, Ursula R. Kees, Nadia Milech |
| المصدر: | Genes, Chromosomes and Cancer. 32:275-280 |
| بيانات النشر: | Wiley, 2001. |
| سنة النشر: | 2001 |
| مصطلحات موضوعية: | Homeodomain Proteins, Genetics, Gene isoform, Cancer Research, Molecular Sequence Data, fungi, Chromosomal translocation, Biology, medicine.disease, Homology (biology), Leukemia, Cell culture, Precursor B-Cell Lymphoblastic Leukemia-Lymphoma, Proto-Oncogene Proteins, medicine, Transcriptional regulation, Humans, Protein Isoforms, Homeobox, splice, Transcription Factors |
| الوصف: | PBX3 is a member of the PBX family of TALE homeobox genes. The prototypic member, PBX1, was first identified in chromosomal translocations in B-lineage leukemia and is required for normal hematopoiesis. PBX2 and PBX3 were later identified as members of this highly conserved family by their strong homology to PBX1. While the expression pattern of PBX1 is restricted, PBX2 and PBX3 are ubiquitously expressed. Little is known about the functional role of PBX3. Our studies identified two PBX3 transcripts alternative to the canonical forms, PBX3A and PBX3B, resulting from a novel splice in PBX3. These new isoforms, named PBX3C and PBX3D, have been detected in all tissues and cell lines tested. Intriguingly, expression of PBX3D is favored in normal cells, whereas PBX3C expression is favored in leukemia cells. Functional studies showed that PBX3C and PBX3D proteins were unable to interact with the PBX-interacting factor PREP1 and weakly interacted with MEIS proteins. We propose that PBX3C and PBX3D may affect PBX3-mediated transcriptional regulation by acting in opposition to the known PBX proteins through alternative PBX3 complex formation. The identification and characterization of these novel PBX3 isoforms provide a foundation for a better understanding of the biological role of PBX3. © 2001 Wiley-Liss, Inc. |
| تدمد: | 1098-2264 1045-2257 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::318bfe1262e7f8aca388bd10e70d0cd9 https://doi.org/10.1002/gcc.1190 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....318bfe1262e7f8aca388bd10e70d0cd9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10982264 10452257 |
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