Circular RNA hsa_circ_0002360 promotes non-small cell lung cancer progression through upregulating matrix metalloproteinase 16 and sponging multiple micorRNAs
| العنوان: | Circular RNA hsa_circ_0002360 promotes non-small cell lung cancer progression through upregulating matrix metalloproteinase 16 and sponging multiple micorRNAs |
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| المؤلفون: | Shaolin Zeng, Yunting Zhang, Tao Wang |
| المصدر: | Bioengineered, Vol 12, Iss 2, Pp 12767-12777 (2021) Bioengineered article-version (VoR) Version of Record |
| بيانات النشر: | Informa UK Limited, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Lung Neoplasms, matrix metalloproteinase 16, Cell, Bioengineering, Matrix metalloproteinase, Biology, Applied Microbiology and Biotechnology, circ_0002360, Cell Movement, Circular RNA, Carcinoma, Non-Small-Cell Lung, Cell Line, Tumor, microRNA, medicine, Humans, Neoplasm Invasiveness, micrornas, non-small cell lung cancer, Reporter gene, Base Sequence, Microarray analysis techniques, RNA, Circular, General Medicine, Middle Aged, Up-Regulation, respiratory tract diseases, Gene Expression Regulation, Neoplastic, medicine.anatomical_structure, Cell culture, Disease Progression, Cancer research, MMP16, TP248.13-248.65, Research Article, Research Paper, Biotechnology |
| الوصف: | Dysregulated circular RNAs (circRNAs) are involved in the progression of non-small cell lung cancer (NSCLC). However, the role of has_circ_0002360 (circ_0002360) in NSCLC has rarely been reported. In this study, circ_0002360 expression in NSCLC tissues and cell lines was measured using microarray data and quantitative real-time PCR (qRT-PCR). After gain-of-function and loss-of-function, cell models were established; 5-bromo-2-deoxyuridine (BrdU) and transwell assays were conducted to detect NSCLC cell growth, migration, and invasion. What is more, bioinformatic analysis and dual-luciferase reporter assay were adopted to show how circ_0002360, microRNAs (miR-127-5p, miR-145-5p, miR-585-3p, and miR-758-3p), and matrix metalloproteinase 16 (MMP16) 3ʹUTR interact with each other. Western blotting was executed to probe the regulatory effects of circ_0002360 and these miRNAs on MMP16 protein expression in NSCLC cells. We found that circ_0002360 expression was raised in NSCLC tissues. High circ_0002360 expression predicted a short overall survival time for NSCLC patients. Circ_0002360 overexpression promoted NSCLC cell proliferative, migrative, and invasive abilities, and circ_0002360 depletion worked oppositely. MiR-127-5p, miR-145-5p, miR-585-3p, and miR-758-3p were the targets of circ_0002360, and circ_0002360 could regulate MMP16 expression by competitively binding with the above miRNAs. In summary, circ_0002360 serves as a competitive endogenous RNA to raise MMP16 expressions by competitively binding to miR-127-5p, miR-145-5p, miR-585-3p, and miR-758-3p, thereby promoting NSCLC progression. Graphical Abstract |
| تدمد: | 2165-5987 2165-5979 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::31f7e4cdd7cdcabdf87caa35f4e50b91 https://doi.org/10.1080/21655979.2021.1999370 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....31f7e4cdd7cdcabdf87caa35f4e50b91 |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 21655987 21655979 |
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