Isolation and Characterization of Desulfitobacterium sp. strain Y51 Capable of Efficient Dehalogenation of Tetrachloroethene and Polychloroethanes
| العنوان: | Isolation and Characterization of Desulfitobacterium sp. strain Y51 Capable of Efficient Dehalogenation of Tetrachloroethene and Polychloroethanes |
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| المؤلفون: | Takashi Tokunaga, Kensuke Furukawa, Akiko Suyama, Nobuyuki Sera, Keiichirou Kai, Ryo Iwakiri |
| المصدر: | Bioscience, Biotechnology, and Biochemistry. 65:1474-1481 |
| بيانات النشر: | Oxford University Press (OUP), 2001. |
| سنة النشر: | 2001 |
| مصطلحات موضوعية: | DNA, Bacterial, Tetrachloroethylene, Stereochemistry, DNA, Ribosomal, Applied Microbiology and Biotechnology, Biochemistry, Analytical Chemistry, chemistry.chemical_compound, Sulfite, Hydrocarbons, Chlorinated, Organic chemistry, Nitrite, Bacillaceae, Molecular Biology, Desulfitobacterium, Soil Microbiology, chemistry.chemical_classification, Ethane, Base Sequence, Strain (chemistry), biology, Chemistry, Organic Chemistry, Desulfitobacterium hafniense, General Medicine, Electron acceptor, biology.organism_classification, HEXA, Microscopy, Electron, Oxidoreductases, Bacteria, Biotechnology |
| الوصف: | A strict anaerobic bacterium, strain Y51, was isolated from soil contaminated with tetrachloroethene (PCE). Strain Y51 is capable of very efficiently dehalogenating PCE via trichloroethene (TCE) to cis-1,2-dichloroethene (cis-1,2-DCE) at concentrations as high as 960 microM and as low as 0.6 microM. Strain Y51 was gram-negative, motile with some lateral flagella, and curved rod-shaped. On the basis of the 16S rDNA sequence, the organism was identified to be a species within the genus Desulfitobacterium. Strain Y51 also had dehalogenation activities toward polychloroethanes such as hexa-, penta-, and tetrachloroethanes, from which dichloroethenes were produced as the final products. The cell extracts mediated the dehalogenation of PCE with reduced methyl viologen as an electron carrier at the specific rate of 5.0 nmol min(-1) mg cell protein(-1) (pH 7.2, 37 degrees C). Dehalogenation was highly susceptible to air oxidation, and to potential alternative electron acceptors such as nitrite or sulfite. |
| تدمد: | 1347-6947 0916-8451 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::322a3144a9dcfe9a3323aa9a1ae0a0ce https://doi.org/10.1271/bbb.65.1474 |
| رقم الأكسشن: | edsair.doi.dedup.....322a3144a9dcfe9a3323aa9a1ae0a0ce |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 13476947 09168451 |
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