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Comparison Of Four Anti-Avian IgY Secondary Antibodies Used In Western Blot And Dot-Blot ELISA To Detect Avian Bornavirus Antibodies In Four Different Bird Species

التفاصيل البيبلوغرافية
العنوان: Comparison Of Four Anti-Avian IgY Secondary Antibodies Used In Western Blot And Dot-Blot ELISA To Detect Avian Bornavirus Antibodies In Four Different Bird Species
المؤلفون: J. Jill Heatley, Ian Tizard, Paulina Escandon, Jeffrey M. B. Musser, Luc Berghman
المصدر: Veterinary Medicine : Research and Reports
Veterinary Medicine: Research and Reports
سنة النشر: 2019
مصطلحات موضوعية: animal structures, 040301 veterinary sciences, Dot blot, serology, Serology, 0403 veterinary science, Macaw, Proventricular dilatation disease, Western blot, biology.animal, medicine, proventricular dilatation disease, Original Research, biology, medicine.diagnostic_test, immunodiagnostics, 0402 animal and dairy science, Research and Reports [Veterinary Medicine], avian ganglioneuritis, 04 agricultural and veterinary sciences, Parakeet, biology.organism_classification, 040201 dairy & animal science, Virology, Primary and secondary antibodies, biology.protein, Antibody
الوصف: Paulina Escandon,1,2 J Jill Heatley,1,3 Luc R Berghman,2,4 Ian Tizard,1,2 Jeffrey MB Musser1,2 1Schubot Exotic Bird Health Center, College of Veterinary Medicine, Texas A&M University, College Station, TX 77843, USA; 2Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, TX 77843, USA; 3Department of Small Animal Clinical Sciences, College of Veterinary Medicine, Texas A&M University, College Station, TX 77843, USA; 4Department of Poultry Science, College of Agriculture & Life Sciences, Texas A&M University, College Station, TX 77843, USACorrespondence: Jeffrey MB Musser4467 TAMU, VTPB, College of Veterinary Medicine, College Station, TX 77843-4467, USATel +1 979 458 9946Fax +1 979 458 0321Email Jmusser@cvm.tamu.eduPurpose: This study evaluated the specificity of different avian secondary antibodies used in Western blot and dot-blot ELISA to detect avian bornavirus antibodies in bird plasma.Methods: Plasma samples were collected from: two Blue and gold macaws, one positive and one negative for avian bornavirus by RT-PCR; a Cockatiel and a Monk parakeet prior to and following experimental infection; and, two Mallards, one positive and one negative for avian bornavirus by RT-PCR Samples were analyzed by Western blot and dot-blot ELISA that incorporated recombinant avian bornavirus nucleoprotein as the target analyte. Four species-specific anti-IgY secondary antibodies were used in the assays: goat anti-macaw IgY, goat anti-bird IgY, goat anti-duck IgY, and rabbit anti-chicken IgY.Results: In the Western blot, anti-macaw IgY secondary antibody produced strong signals with Blue and gold macaw and Cockatiel positive plasma, but no signal with Mallard positive plasma. Anti-bird IgY secondary antibody produced strong signals with Blue and gold macaw, Cockatiel, and Mallard positive plasma. Anti-duck and anti-chicken IgY secondary antibody produced a strong and moderate signal, respectively, only with Mallard positive plasma. In the dot-blot ELISA, there was a distinct and significant difference (P
وصف الملف: text/html
تدمد: 2230-2034
7843-4467
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3236e06ac07f863f2652c49fb0834bde
https://pubmed.ncbi.nlm.nih.gov/31815098
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....3236e06ac07f863f2652c49fb0834bde
قاعدة البيانات: OpenAIRE
الوصف
تدمد:22302034
78434467