Engineering the Yeast Saccharomyces cerevisiae for the Production of L-(+)-Ergothioneine
| العنوان: | Engineering the Yeast Saccharomyces cerevisiae for the Production of L-(+)-Ergothioneine |
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| المؤلفون: | Bala K. Prabhala, Douglas B. Kell, Behrooz Darbani, Steven Axel van der Hoek, Jacqueline B. Medina, Irina Borodina, Mathias Bernfried Biron, Karolina E. Zugaj, Milica Randelovic |
| المصدر: | Frontiers in Bioengineering and Biotechnology Frontiers in Bioengineering and Biotechnology, Vol 7 (2019) van der Hoek, S A, Darbani, B, Zugaj, K E, Prabhala, B K, Biron, M B, Randelovic, M, Medina, J B, Kell, D B & Borodina, I 2019, ' Engineering the Yeast Saccharomyces cerevisiae for the Production of L-(+)-Ergothioneine ', Frontiers in Bioengineering and Biotechnology, vol. 7, 262 . https://doi.org/10.3389/fbioe.2019.00262 |
| بيانات النشر: | Frontiers Media SA, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Antioxidant, Histology, lcsh:Biotechnology, medicine.medical_treatment, Saccharomyces cerevisiae, Biomedical Engineering, Bioengineering, 02 engineering and technology, yeast, Metabolic engineering, 03 medical and health sciences, chemistry.chemical_compound, Nutraceutical, lcsh:TP248.13-248.65, Generally recognized as safe, Bioreactor, medicine, 030304 developmental biology, 2. Zero hunger, 0303 health sciences, biology, 030302 biochemistry & molecular biology, ergothioneine, 021001 nanoscience & nanotechnology, biology.organism_classification, Yeast, Chemically defined medium, 030104 developmental biology, Biochemistry, chemistry, Ergothioneine, medium optimization, Fermentation, nutraceutical, metabolic engineering, 0210 nano-technology, Erg, Biotechnology |
| الوصف: | L-(+)-Ergothioneine is an unusual, naturally occurring antioxidant nutraceutical that has been shown to help reduce cellular oxidative damage. Humans do not biosynthesise it, so can acquire it only from their diet; it exploits a specific transporter (SLC22A4) for its uptake. ERG is considered to be a nutraceutical and possible vitamin that is involved in the maintenance of health, and seems to be at too low a concentration in several diseasesin vivo. Ergothioneine is thus a potentially useful dietary supplement. Present methods of commercial production rely on extraction from natural sources or on chemical synthesis. Here we describe the engineering of the baker’s yeastSaccharomyces cerevisiaeto produce ergothioneine by fermentation in defined media. After integrating combinations of ERG biosynthetic pathways from different organisms, we screened yeast strains for their production of ERG. The highest-producing strain was also engineered with known ergothioneine transporters. The effect of amino acid supplementation of the medium was investigated and the nitrogen metabolism ofS. cerevisiaewas altered by knock-out ofTOR1 orYIH1. We also optimized the media composition using fractional factorial methods. Our optimal strategy led to a titer of 598 ± 18 mg/L ergothioneine in fed-batch culture in bioreactors. BecauseS. cerevisiaeis a GRAS (‘generally recognised as safe’) organism that is widely used for nutraceutical production, this work provides a promising process for the biosynthetic production of ERG. |
| وصف الملف: | application/pdf |
| تدمد: | 2296-4185 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::323ceeb7f8d9222ca59bcc5883e53a0a https://doi.org/10.3389/fbioe.2019.00262 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....323ceeb7f8d9222ca59bcc5883e53a0a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22964185 |
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