Different regulation of PARP1, PARP2, PARP3 and TRPM2 genes expression in acute myeloid leukemia cells
| العنوان: | Different regulation of PARP1, PARP2, PARP3 and TRPM2 genes expression in acute myeloid leukemia cells |
|---|---|
| المؤلفون: | Mansur Rahnama, Janusz Kocki, Wojciech Kocki, Marcin Feldo, Anna Bogucka-Kocka, Genowefa Anna Wawer, Joanna Wawer, Paulina Gil-Kulik, Elżbieta Radzikowska-Büchner, Adam Nogalski, Mariusz Jojczuk, Maria Cioch, Ewa Dudzińska |
| المصدر: | BMC Cancer BMC Cancer, Vol 20, Iss 1, Pp 1-9 (2020) |
| بيانات النشر: | BioMed Central, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Adult, Male, Cancer Research, Poly (ADP-Ribose) Polymerase-1, TRPM Cation Channels, Bone Marrow Cells, Cell Cycle Proteins, Biology, lcsh:RC254-282, Peripheral blood mononuclear cell, PARP1, PARP2, PARP3, 03 medical and health sciences, Young Adult, 0302 clinical medicine, AML, hemic and lymphatic diseases, Gene expression, Genetics, medicine, Biomarkers, Tumor, Humans, Aged, TRPM2 gene expression, Myeloid leukemia, Cancer, Middle Aged, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, medicine.disease, Hematopoietic Stem Cells, Prognosis, Gene Expression Regulation, Neoplastic, Haematopoiesis, Leukemia, Myeloid, Acute, 030104 developmental biology, medicine.anatomical_structure, Oncology, Tumor progression, 030220 oncology & carcinogenesis, Case-Control Studies, Cancer research, Female, Bone marrow, Stem cell, Poly(ADP-ribose) Polymerases, Research Article, Follow-Up Studies |
| الوصف: | Background Acute myeloid leukemia (AML) is a heterogenic lethal disorder characterized by the accumulation of abnormal myeloid progenitor cells in the bone marrow which results in hematopoietic failure. Despite various efforts in detection and treatment, many patients with AML die of this cancer. That is why it is important to develop novel therapeutic options, employing strategic target genes involved in apoptosis and tumor progression. Methods The aim of the study was to evaluate PARP1, PARP2, PARP3, and TRPM2 gene expression at mRNA level using qPCR method in the cells of hematopoietic system of the bone marrow in patients with acute myeloid leukemia, bone marrow collected from healthy patients, peripheral blood of healthy individuals, and hematopoietic stem cells from the peripheral blood after mobilization. Results The results found that the bone marrow cells of the patients with acute myeloid leukemia (AML) show overexpression of PARP1 and PARP2 genes and decreased TRPM2 gene expression. In the hematopoietic stem cells derived from the normal marrow and peripheral blood after mobilization, the opposite situation was observed, i.e. TRPM2 gene showed increased expression while PARP1 and PARP2 gene expression was reduced. We observed positive correlations between PARP1, PARP2, PARP3, and TRPM2 genes expression in the group of mature mononuclear cells derived from the peripheral blood and in the group of bone marrow-derived cells. In AML cells significant correlations were not observed between the expression of the examined genes. In addition, we observed that the reduced expression of TRPM2 and overexpression of PARP1 are associated with a shorter overall survival of patients, indicating the prognostic significance of these genes expression in AML. Conclusions Our research suggests that in physiological conditions in the cells of the hematopoietic system there is mutual positive regulation of PARP1, PARP2, PARP3, and TRPM2 genes expression. PARP1, PARP2, and TRPM2 genes at mRNA level deregulate in acute myeloid leukemia cells. |
| اللغة: | English |
| تدمد: | 1471-2407 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::324bc4481c17b5f656295fe6e4b26ba3 http://europepmc.org/articles/PMC7236215 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....324bc4481c17b5f656295fe6e4b26ba3 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14712407 |
|---|