MicroRNAs secreted by human embryos could be potential biomarkers for clinical outcomes of assisted reproductive technology
| العنوان: | MicroRNAs secreted by human embryos could be potential biomarkers for clinical outcomes of assisted reproductive technology |
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| المؤلفون: | Jiangyu Yu, Wenpei Xiang, Fang Fang, Zili Li, Xiaofang Ding, Shumin Shao, Li Wu, Qian Zhao, Yuting Long, Ling Zhang |
| المصدر: | Journal of Advanced Research, Vol 31, Iss, Pp 25-34 (2021) Journal of Advanced Research |
| بيانات النشر: | Elsevier, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Adult, medicine.medical_specialty, Medicine (General), Science (General), Reproductive Techniques, Assisted, medicine.medical_treatment, Cleavage Stage, Ovum, Reproductive medicine, Biology, Real-Time Polymerase Chain Reaction, law.invention, Andrology, 03 medical and health sciences, Q1-390, 0302 clinical medicine, R5-920, law, Pregnancy, Embryo culture media, microRNA, medicine, Humans, Digital polymerase chain reaction, Embryo Implantation, Polymerase chain reaction, ComputingMethodologies_COMPUTERGRAPHICS, Multidisciplinary, Assisted reproductive technology, Computational Biology, Embryo, medicine.disease, Embryo, Mammalian, Embryonic stem cell, Culture Media, Reproductive outcome, MicroRNAs, 030104 developmental biology, ROC Curve, Reproductive Medicine, 030220 oncology & carcinogenesis, embryonic structures, Medicine, Female, Biomarkers |
| الوصف: | Graphical abstract Introduction MicroRNAs (miRNAs) are important regulators of many biological functions, including embryo implantation and development. Recently, it has been reported that miRNAs in biofluids are predictive for physiological and pathological processes. Objectives In this study, we aim to investigate whether the miRNAs secreted by human embryos in culture medium can be used as embryonic biomarkers. Methods The culture media were prospectively collected from embryos of patients at reproductive medicine center with informed consent. A high-throughput miRNA sequencing method was applied to detect the miRNA profiles in the human embryo culture media. After bioinformatics analysis and screening of differentially expressed miRNAs, quantitative real-time polymerase chain reaction (qRT-PCR) assay was subsequently performed to further confirm the sequencing results with mixed samples. Furthermore, we performed droplet digital PCR (ddPCR) to verify the target miRNAs at single sample level. Receiver operating characteristic (ROC) analyses were performed for differentially expressed miRNAs. Results Compared with embryos with failed pregnancy, the embryos with successful pregnancy secreted different miRNA profiles into the culture media, which were predicted to be involved in multiple biological processes. Validated by droplet digital polymerase chain reaction (ddPCR), the expression of hsa-miR-26b-5p and hsa-miR-21-5p in the culture media of cleavage embryos with successful pregnancy was significantly lower than that of embryos with failed pregnancy. Moreover, the Receiver Operating Characteristic (ROC) curve analysis indicated that hsa-miR-26b-5p and hsa-miR-21-5p could serve as potential biomarkers for reproductive outcomes. Conclusion Together, our findings highlight the important predictive potential of miRNAs secreted by human embryos in culture media, which is meaningful for non-invasive embryo selection in assisted reproductive technology. |
| اللغة: | English |
| تدمد: | 2090-1232 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3284cb2a8e0ad607689f42bf8fe714ad http://www.sciencedirect.com/science/article/pii/S2090123221000035 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....3284cb2a8e0ad607689f42bf8fe714ad |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20901232 |
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