LncRNA DQ786243 contributes to proliferation and metastasis of colorectal cancer both in vitro and in vivo
| العنوان: | LncRNA DQ786243 contributes to proliferation and metastasis of colorectal cancer both in vitro and in vivo |
|---|---|
| المؤلفون: | Hong Zhou, Lei Gu, Hanbing Xue, Qing Xu, Chunjie Xu, Ye Liu, Longci Sun, Chunhui Jiang |
| المصدر: | Bioscience Reports |
| بيانات النشر: | Portland Press Ltd., 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Expression of Concern, 0301 basic medicine, Colon, Cell, Biophysics, Mice, Nude, Biology, migration, colorectal cancer (CRC), Biochemistry, 03 medical and health sciences, 0302 clinical medicine, Cell Movement, RNA interference, Cell Line, Tumor, medicine, Animals, Humans, Gene silencing, Neoplasm Invasiveness, Neoplasm Metastasis, Molecular Biology, Cell Proliferation, long non-coding ribonucleic acid (LncRA), Original Paper, Mice, Inbred BALB C, Gene knockdown, Oncogene, Rectum, Cell Biology, S16, Cell cycle, invasion, Original Papers, Molecular biology, digestive system diseases, Long non-coding RNA, Gene Expression Regulation, Neoplastic, long non-coding ribonucleic acid (LncRNA), 030104 developmental biology, medicine.anatomical_structure, S39, Tumor progression, 030220 oncology & carcinogenesis, Cancer research, RNA, Long Noncoding, Colorectal Neoplasms |
| الوصف: | This article aims to find the key long non-coding RNAs (LncRNAs) associated with colorectal cancer (CRC) and to study its biological functions in colorectal cancer progression. Our study has shown that upregulated LncRNA DQ786243 can regulate cell proliferation, cell cycle progression, cell apoptosis, migration, and invasion in CRC cells. Xenograft experiments confirmed that the growth of xenograft tumors formed by CRC cells was suppressed after silencing LncRNA DQ786243 expression. In conclusion, our study suggests that LncRNA DQ786243 is an oncogene that promotes tumor progression and leads us to propose that LncRNAs may serve as key regulatory hubs in CRC progression. Accumulating evidence demonstrates that long non-coding RNAs (LncRNAs) play important roles in regulating gene expression and are involved in various cancers, including colorectal cancer (CRC). However, LncRNA profiles in CRC remain largely unknown. The present study aims to find the key LncRNA associated with CRC and to study its biological functions in CRC progression. We focused on LncRNA DQ786243, one of LncRNAs which promoted development of CRC from the Gene Expression Omnibus (GEO) and validated using quantitative real-time PCR among about 20 paired CRC tissues. The effects of LncRNA DQ786243 were assessed by silencing the LncRNA in vitro and in vivo. Results showed that the expression level LncRNA DQ786243 was significantly higher in CRC tissues and cell lines. We also found LncRNA DQ786243 knockdown by RNA interference with siRNA significantly arrested the cell cycle in the G2/M-phase, promoted apoptosis and weaken the abilities of cell proliferation and invasion in vitro. Further investigation into the mechanisms responsible for the growth inhibitory effects by DQ786243 silencing revealed that its knockdown resulted in the induction of cell cycle arrest and apoptosis through certain cell cycle-related and apoptosis-related proteins. Finally, xenograft experiments confirmed that the growth of xenograft tumours formed by CRC cells was suppressed after silencing LncRNA DQ786243 expression. In conclusion, the present study suggests that LncRNA DQ786243 is an oncogene that promotes tumour progression and leads us to propose that LncRNAs may serve as key regulatory hubs in CRC progression. |
| تدمد: | 1573-4935 0144-8463 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3293404311856bc1ebed2944eed7517a https://doi.org/10.1042/bsr20160048 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....3293404311856bc1ebed2944eed7517a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15734935 01448463 |
|---|