Cytosolic proteome profiling of monocytes for male osteoporosis
| العنوان: | Cytosolic proteome profiling of monocytes for male osteoporosis |
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| المؤلفون: | Hong-Wen Deng, Yong Zeng, Zhao Yc, Ji-Gang Zhang, Huang Hl, Lan-Juan Zhao, Lei Zhang, Hui Shen, Wei Zhu, Qing Tian, Kehao Wu, Hao He, Fei-Yan Deng, Yu Zhou |
| المصدر: | Osteoporosis International. 28:1035-1046 |
| بيانات النشر: | Springer Science and Business Media LLC, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Adult, Male, Proteomics, musculoskeletal diseases, 0301 basic medicine, medicine.medical_specialty, Proteome, Endocrinology, Diabetes and Metabolism, Osteoporosis, Carbohydrate metabolism, Monocytes, Article, 03 medical and health sciences, Absorptiometry, Photon, Cytosol, Bone Density, Fructose-Bisphosphate Aldolase, Internal medicine, medicine, Humans, Gene Regulatory Networks, Myosin Type II, Myosin Heavy Chains, business.industry, Gene Expression Profiling, Middle Aged, Omics, medicine.disease, Rheumatology, Gene Ontology, rap GTP-Binding Proteins, 030104 developmental biology, Mineral density, Endocrinology, Proteome profiling, Gene Expression Regulation, Immunology, business |
| الوصف: | In male Caucasians with discordant hip bone mineral density (BMD), we applied the subcellular separation and proteome profiling to investigate the monocytic cytosol. Three BMD-associated proteins (ALDOA, MYH14, and Rap1B) were identified based on multiple omics evidence, and they may influence the pathogenic mechanisms of osteoporosis by regulating the activities of monocytes.Osteoporosis is a serious public health problem, leading to significant mortality not only in aging females but also in males. Peripheral blood monocytes (PBMs) play important roles in bone metabolism by acting as precursors of osteoclasts and producing cytokines important for osteoclast development. The first cytosolic sub-proteome profiling analysis was performed in male PBMs to identify differentially expressed proteins (DEPs) that are associated with BMDs and risk of osteoporosis.Here, we conducted a comparative proteomics analysis in PBMs from Caucasian male subjects with discordant hip BMD (29 low BMD vs. 30 high BMD). To decrease the proteome complexity and expand the coverage range of the cellular proteome, we separated the PBM proteome into several subcellular compartments and focused on the cytosolic fractions, which are involved in a wide range of fundamental biochemical processes.Of the total of 3796 detected cytosolic proteins, we identified 16 significant (P 0.05) and an additional 22 suggestive (P 0.1) DEPs between samples with low vs. high hip BMDs. Some of the genes for DEPs, including ALDOA, MYH14, and Rap1B, showed an association with BMD in multiple omics studies (proteomic, transcriptomic, and genomic). Further bioinformatics analysis revealed the enrichment of DEPs in functional terms for monocyte proliferation, differentiation, and migration.The combination strategy of subcellular separation and proteome profiling allows an in-depth and refined investigation into the composition and functions of cytosolic proteome, which may shed light on the monocyte-mediated pathogenic mechanisms of osteoporosis. |
| تدمد: | 1433-2965 0937-941X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::32a697bdb53d26856443f2be4abb0072 https://doi.org/10.1007/s00198-016-3825-y |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....32a697bdb53d26856443f2be4abb0072 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14332965 0937941X |
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