Rapid Optical Cavity PCR
| العنوان: | Rapid Optical Cavity PCR |
|---|---|
| المؤلفون: | Jun Ho Son, Minsun Song, Luke P. Lee, Amanda J. Haack, Ori Hoxha, SoonGweon Hong, Lars Gustafson |
| المصدر: | Advanced healthcare materials, vol 5, iss 1 Advanced Healthcare Materials Advanced Healthcare Materials, vol 5, iss 1 |
| بيانات النشر: | eScholarship, University of California, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Materials science, Optical Phenomena, polymerase chain reaction, light-emitting diodes, Medical Biotechnology, point‐of‐care diagnostics, Analytical chemistry, Biomedical Engineering, Pharmaceutical Science, law.invention, Biomaterials, molecular diagnostics, 03 medical and health sciences, Medicinal and Biomolecular Chemistry, law, Humans, Computer Simulation, Full Paper, Temperature, Full Papers, 030104 developmental biology, Emerging Infectious Diseases, Good Health and Well Being, light‐emitting diodes, Optical cavity, Nucleic acid, Pcr method, Gold, optical cavities, Electronics, point-of-care diagnostics |
| الوصف: | Recent outbreaks of deadly infectious diseases, such as Ebola and Middle East respiratory syndrome coronavirus, have motivated the research for accurate, rapid diagnostics that can be administered at the point of care. Nucleic acid biomarkers for these diseases can be amplified and quantified via polymerase chain reaction (PCR). In order to solve the problems of conventional PCR—speed, uniform heating and cooling, and massive metal heating blocks—an innovative optofluidic cavity PCR method using light‐emitting diodes (LEDs) is accomplished. Using this device, 30 thermal cycles between 94 °C and 68 °C can be accomplished in 4 min for 1.3 μL (10 min for 10 μL). Simulation results show that temperature differences across the 750 μm thick cavity are less than 2 °C and 0.2 °C, respectively, at 94 °C and 68 °C. Nucleic acid concentrations as low as 10–8 ng μL–1 (2 DNA copies per μL) can be amplified with 40 PCR thermal cycles. This simple, ultrafast, precise, robust, and low‐cost optofluidic cavity PCR is favorable for advanced molecular diagnostics and precision medicine. It is especially important for the development of lightweight, point‐of‐care devices for use in both developing and developed countries. Novel optical cavity polymerase chain reaction (PCR) driven by a light‐emitting diode (LED) is demonstrated for fast, accurate, and reliable PCR‐based diagnostics. Using optical cavity PCR, nucleic acid amplification with the template DNA concentration has been successfully demonstrated, ranging from 10−5 to 10−8 ng μL−1 within 5–15 min with excellent repeatability and reproducibility. |
| وصف الملف: | application/pdf |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::335c75b47f82d7e524a82866d431d1d7 https://escholarship.org/uc/item/4r56s1w7 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....335c75b47f82d7e524a82866d431d1d7 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |