Validation of the Soleris®Enterobacteriaceae Method for Detection of Enterobacteriaceae in Dried Cannabis Flower: AOAC Performance Tested MethodSM 121901
| العنوان: | Validation of the Soleris®Enterobacteriaceae Method for Detection of Enterobacteriaceae in Dried Cannabis Flower: AOAC Performance Tested MethodSM 121901 |
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| المؤلفون: | Quynh-Nhi Le, Mark Mozola, Alexandra Tudor, Leo Horine, Brooke Roman, Carolyn Montei, Lei Zhang, Preetha Biswas, Robert Donofrio |
| المصدر: | Journal of AOAC INTERNATIONAL. 105:1671-1676 |
| بيانات النشر: | Oxford University Press (OUP), 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Pharmacology, Enterobacteriaceae, Food Microbiology, Environmental Chemistry, Dronabinol, Flowers, Agronomy and Crop Science, Cannabis, Food Science, Analytical Chemistry |
| الوصف: | Background The Soleris®Enterobacteriaceae vial is a growth-based, automated method for detection of bacteria of the family Enterobacteriaceae in foods and other sample types including nutraceuticals and cosmetics. The Soleris method is used in a “dilute-to-specification” or threshold manner, in which a result is scored as positive or negative around a predetermined cutoff (in CFU/g) established by the dilution and volume of sample homogenate tested. The Soleris method was granted AOAC Performance Tested MethodSM (PTM) status for select foods after successful completion of a validation study (PTM 121901). Objective The objective of this study was to validate the method for the detection of Enterobacteriaceae in dried cannabis flower [>0.3% delta-9-tetrahydrocannabinol (THC)]. Methods The matrix study included comparison of Soleris method presumptive results to confirmation from the Soleris vials, and comparison of the Soleris confirmed results to those of the ISO 21528-2:2017 colony count method. Test materials at four different levels of contamination ranging from 7.8 to 3500 CFU/g were tested at three dilutions, corresponding to test thresholds. Results Probability of detection analysis at P Conclusion The results provided evidence that the Soleris Enterobacteriaceae test is an accurate method for detection of Enterobacteriaceae in dried cannabis flower. Highlights The Soleris Enterobacteriaceae method provides cannabis industry QC personnel with an effective method for analysis of dried cannabis flower and produces results in 20–24 h. |
| تدمد: | 1944-7922 1060-3271 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::339681810647dc1d3329c63b89d1aa8c https://doi.org/10.1093/jaoacint/qsac063 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....339681810647dc1d3329c63b89d1aa8c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19447922 10603271 |
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