Sperm chromatin condensation defects, but neither DNA fragmentation nor aneuploidy, are an independent predictor of clinical pregnancy after intracytoplasmic sperm injection
| العنوان: | Sperm chromatin condensation defects, but neither DNA fragmentation nor aneuploidy, are an independent predictor of clinical pregnancy after intracytoplasmic sperm injection |
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| المؤلفون: | M. Letailleur, Fanny Jumeau, Louis Sibert, Cynthia Bichara, Aurélie Rives, Christine Rondanino, Véronique Sétif, Benoit Berby, Nathalie Rives |
| المساهمون: | Gamétogenèse et Qualité du Gamète - ULR 4308 (GQG), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Université de Lille, CHU Rouen, Normandie Université (NU) |
| المصدر: | Journal of Assisted Reproduction and Genetics Journal of Assisted Reproduction and Genetics, 2019, 36 (7), pp.1387-1399. ⟨10.1007/s10815-019-01471-4⟩ J Assist Reprod Genet Journal of Assisted Reproduction and Genetics, Springer Verlag, 2019, 36 (7), pp.1387-1399. ⟨10.1007/s10815-019-01471-4⟩ |
| بيانات النشر: | HAL CCSD, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Adult, Male, Pregnancy Rate, medicine.medical_treatment, Aneuploidy, Semen, DNA Fragmentation, Fertilization in Vitro, Intracytoplasmic sperm injection, Andrology, 03 medical and health sciences, 0302 clinical medicine, Ovulation Induction, Pregnancy, Gamete Biology, Genetics, medicine, Humans, Sperm Injections, Intracytoplasmic, Spermatogenesis, Genetics (clinical), reproductive and urinary physiology, Infertility, Male, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 0303 health sciences, 030219 obstetrics & reproductive medicine, Sperm chromatin condensation, business.industry, urogenital system, Obstetrics and Gynecology, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, General Medicine, medicine.disease, Sperm, Spermatozoa, Chromatin, Semen Analysis, Pregnancy rate, Reproductive Medicine, Sperm Motility, DNA fragmentation, Female, business, Developmental Biology |
| الوصف: | PURPOSE: The impact of sperm DNA damage on intracytoplasmic sperm injection (ICSI) outcomes remains controversial. The purpose of the study was to evaluate the prognostic value of several types of sperm nuclear damage on ICSI clinical pregnancy. METHODS: Our retrospective study included a total of 132 couples who consulted for male or mixed-factor infertility that benefited from ICSI cycles from January 2006 to December 2015. All infertile males presented at least one conventional semen parameter alteration. Sperm nuclear damage was assessed using the Motile Sperm Organelle Morphological Examination for sperm head relative vacuolar area (RVA), aniline blue staining for chromatin condensation, terminal deoxynucleotidyl transferase dUTP nick-end labeling for DNA fragmentation, and fluorescence in situ hybridization for aneuploidy. RESULTS: Infertile males who achieved pregnancy after ICSI had fewer chromatin condensation defects than did males who did not achieve any pregnancy (15.8 ± 12.0% vs. 11.4 ± 7.9%, respectively, P = 0.0242), which remained significant in multivariate regression analysis (RR = 0.40 [0.18 to 0.86], P = 0.02). RVA, DNA fragmentation, and aneuploidy were not predictive factors of ICSI outcomes. The pregnancy rate was significantly decreased by number of progressive motile spermatozoa with normal morphology after migration (P = 0.04). In female partners, 17β estradiol of less than 2000 pg/mL on the day of ovulation induction significantly reduced the occurrence of clinical pregnancy (P = 0.04). CONCLUSION: Sperm chromatin condensation defects were more frequently observed in couples with ICSI failure and should be considered a negative predictive factor for the occurrence of clinical pregnancy. |
| اللغة: | English |
| تدمد: | 1058-0468 1573-7330 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::33d2203a9d9aacb3ff73e89d1cf9f427 https://hal.archives-ouvertes.fr/hal-02302047 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....33d2203a9d9aacb3ff73e89d1cf9f427 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10580468 15737330 |
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