Ciliary transcription factors and miRNAs precisely regulate Cp110 levels required for ciliary adhesions and ciliogenesis
| العنوان: | Ciliary transcription factors and miRNAs precisely regulate Cp110 levels required for ciliary adhesions and ciliogenesis |
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| المؤلفون: | Chris Kintner, Dingyuan I Sun, Ian K. Quigley, Umeet K Sajjan, Peter Walentek, Richard M. Harland |
| المصدر: | Walentek, P; Quigley, IK; Sun, DI; Sajjan, UK; Kintner, C; & Harland, RM. (2016). Ciliary transcription factors and miRNAs precisely regulate Cp110 levels required for ciliary adhesions and ciliogenesis. eLife, 5(September2016). doi: 10.7554/eLife.17557. UC Berkeley: Retrieved from: http://www.escholarship.org/uc/item/0ck4x2p8 eLife, vol 5, iss September2016 eLife eLife, Vol 5 (2016) |
| بيانات النشر: | eScholarship, University of California, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Centriole, QH301-705.5, Science, Xenopus, 1.1 Normal biological development and functioning, Biology, General Biochemistry, Genetics and Molecular Biology, Focal adhesion, 03 medical and health sciences, developmental biology, stem cells, Underpinning research, Ciliogenesis, cell biology, Genetics, Basal body, centriole, human, Biology (General), focal adhesion, Transcription factor, mucociliary epithelia, Actin, MCIDAS, General Immunology and Microbiology, miR-34, General Neuroscience, Cilium, cilia, miR-449, General Medicine, Cell Biology, biology.organism_classification, xenopus, Cell biology, RFX2, Foxj1, 030104 developmental biology, Developmental Biology and Stem Cells, Medicine, Generic health relevance, Biochemistry and Cell Biology, Research Article, Human |
| الوصف: | Upon cell cycle exit, centriole-to-basal body transition facilitates cilia formation. The centriolar protein Cp110 is a regulator of this process and cilia inhibitor, but its positive roles in ciliogenesis remain poorly understood. Using Xenopus we show that Cp110 inhibits cilia formation at high levels, while optimal levels promote ciliogenesis. Cp110 localizes to cilia-forming basal bodies and rootlets, and is required for ciliary adhesion complexes that facilitate Actin interactions. The opposing roles of Cp110 in ciliation are generated in part by coiled-coil domains that mediate preferential binding to centrioles over rootlets. Because of its dual role in ciliogenesis, Cp110 levels must be precisely controlled. In multiciliated cells, this is achieved by both transcriptional and post-transcriptional regulation through ciliary transcription factors and microRNAs, which activate and repress cp110 to produce optimal Cp110 levels during ciliogenesis. Our data provide novel insights into how Cp110 and its regulation contribute to development and cell function. DOI: http://dx.doi.org/10.7554/eLife.17557.001 |
| وصف الملف: | application/pdf |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::33d2c9b5d2127906b42051138c84162c http://www.escholarship.org/uc/item/0ck4x2p8 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....33d2c9b5d2127906b42051138c84162c |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |