Gene Expression Analysis of Human Papillomavirus-Associated Colorectal Carcinoma
| العنوان: | Gene Expression Analysis of Human Papillomavirus-Associated Colorectal Carcinoma |
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| المؤلفون: | Xiaoyang Jiao, Leshan Cai, Xiang Chen, Kaixi Liu, Qiancheng Qiu, Zhiqiang Fan, Yumeng Yuan, Wenjun Shen, Yazhen Li, Fen Yao, Jiayu Sun, Jinghong Chen, Yanxuan Xie |
| المصدر: | BioMed Research International BioMed Research International, Vol 2020 (2020) |
| بيانات النشر: | Hindawi Limited, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Adult, Male, 0301 basic medicine, Article Subject, Colorectal cancer, Biology, medicine.disease_cause, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, 0302 clinical medicine, Antigen, medicine, AXIN2, Humans, Papillomaviridae, Gene, Aged, Aged, 80 and over, General Immunology and Microbiology, Oncogene, Papillomavirus Infections, HPV infection, virus diseases, General Medicine, Middle Aged, medicine.disease, Neoplasm Proteins, Gene Expression Regulation, Neoplastic, 030104 developmental biology, 030220 oncology & carcinogenesis, Cancer research, Medicine, Immunohistochemistry, Female, Colorectal Neoplasms, Carcinogenesis, Research Article |
| الوصف: | Purpose. Human papillomavirus (HPV) antigens had been found in colorectal cancer (CRC) tissue, but little evidence demonstrates the association of HPV with oncogene mutations in CRC. We aim to elucidate the mutated genes that link HPV infection and CRC carcinogenesis. Methods. Cancerous and adjacent noncancerous tissues were obtained from CRC patients. HPV antigen was measured by using the immunohistochemical (IHC) technique. The differentially expressed genes (DEGs) in HPV-positive and HPV-negative tumor tissues were measured by using TaqMan Array Plates. The target genes were validated with the qPCR method. Results. 15 (31.9%) cases of CRC patients were observed to be HPV positive, in which HPV antigen was expressed in most tumor tissues rather than in adjacent noncancerous tissues. With TaqMan Array Plates analyses, we found that 39 differentially expressed genes (DEGs) were upregulated, while 17 DEGs were downregulated in HPV-positive CRC tissues compared with HPV-negative tissues. Four DEGs (MMP-7, MYC, WNT-5A, and AXIN2) were upregulated in tumor vs. normal tissues, or adenoma vs. normal tissue in TCGA, which was overlapped with our data. In the confirmation test, MMP-7, MYC, WNT-5A, and AXIN2 were upregulated in cancerous tissue compared with adjacent noncancerous tissue. MYC, WNT-5A, and AXIN2 were shown to be upregulated in HPV-positive CRC tissues when compared to HPV-negative tissues. Conclusion. HPV-encoding genome may integrate into the tumor genomes that involved in multiple signaling pathways. Further genomic and proteomic investigation is necessary for obtaining a more comprehensive knowledge of signaling pathways associated with the CRC carcinogenesis. |
| وصف الملف: | text/xhtml |
| تدمد: | 2314-6141 2314-6133 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::34320c8072049db2528a89622cb5f9cb https://doi.org/10.1155/2020/5201587 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....34320c8072049db2528a89622cb5f9cb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23146141 23146133 |
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