Additional file 1: Figure S1. BSEP is retained at aberrant CHMP5-positive subapical compartments in a transient cholestatic human liver sample. Figure S2. The total membrane-protein fraction contains the plasma-membrane plus organelle-membrane protein fractions. Figure S3. The ESCRT-III subunits CHMP5 and LIP5 co-localize with BSEP-resident subapical compartments in adult human hepatocytes. Figure S4. The canalicular targeting of BSEP is developmentally regulated and associated with CHMP5 in human livers. Figure S5. The protein expression and turnover of BSEP is unaffected with CHMP5 knockdown. Figure S6. Both VPS4A and VPS4B affect post-Golgi trafficking of BSEP.