Direct Evidence for Requirement of Phosphatidylglycerol in Photosystem II of Photosynthesis
| العنوان: | Direct Evidence for Requirement of Phosphatidylglycerol in Photosystem II of Photosynthesis |
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| المؤلفون: | Miki Hagio, Zsuzsanna Várkonyi, Hajime Wada, Norihiro Sato, Kazumori Masamoto, Zoltán Gombos, Mikio Tsuzuki |
| المصدر: | Plant Physiology. 124:795-804 |
| بيانات النشر: | Oxford University Press (OUP), 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | Photosystem II, Physiology, Molecular Sequence Data, Photosynthetic Reaction Center Complex Proteins, Mutant, Transferases (Other Substituted Phosphate Groups), Plant Science, Cyanobacteria, Photosynthesis, chemistry.chemical_compound, Genetics, Amino Acid Sequence, DNA Primers, Photosystem, Phosphatidylglycerol, Base Sequence, Sequence Homology, Amino Acid, ATP synthase, biology, Synechocystis, Phosphatidylglycerols, biology.organism_classification, chemistry, Biochemistry, Genes, Bacterial, Thylakoid, Mutation, biology.protein, Research Article |
| الوصف: | Phosphatidylglycerol (PG) is considered to play an important role in the ordered assembly and structural maintenance of the photosynthetic apparatus in thylakoid membranes. However, its function in photosynthesis remains poorly understood. In this study we have identified a pgsA gene of Synechocystissp. PCC6803 that encodes a PG phosphate synthase involved in the biosynthesis of PG. A disruption of the pgsA gene allowed us to manipulate the content of PG in thylakoid membranes and to investigate the function of PG in photosynthesis. The obtainedpgsA mutant could grow only in the medium containing PG, and the photosynthetic activity of the pgsA mutant dramatically decreased with a concomitant decrease of PG content in thylakoid membranes when the cells grown in the presence of PG were transferred to the medium without PG. This decrease of photosynthetic activity was attributed to the decrease of photosystem (PS)II activity, but not to the decrease in PSI activity. These findings demonstrate that PG is essential for growth of Synechocystis sp. PCC6803 and provide the first direct evidence that PG plays an important role in PSII. |
| تدمد: | 1532-2548 0032-0889 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::34a55c16aaee103e8c7dfbf82758c0f4 https://doi.org/10.1104/pp.124.2.795 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....34a55c16aaee103e8c7dfbf82758c0f4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15322548 00320889 |
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