MIV-150/Zinc Acetate Gel Inhibits Cell-Associated Simian-Human Immunodeficiency Virus Reverse Transcriptase Infection in a Macaque Vaginal Explant Model
| العنوان: | MIV-150/Zinc Acetate Gel Inhibits Cell-Associated Simian-Human Immunodeficiency Virus Reverse Transcriptase Infection in a Macaque Vaginal Explant Model |
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| المؤلفون: | Radhika Menon, Giulia Calenda, Thierry Bonnaire, Michael L. Cooney, Natalia Teleshova, Keith Levendosky, Patrick Barnable, Thomas M. Zydowsky, Agegnehu Gettie, James Blanchard, José A. Fernández-Romero |
| المصدر: | Antimicrobial Agents and Chemotherapy. 59:3829-3837 |
| بيانات النشر: | American Society for Microbiology, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Pyridines, Simian Acquired Immunodeficiency Syndrome, Zinc Acetate, Cervix Uteri, Biology, medicine.disease_cause, Antiviral Agents, Macaque, Microbicide, biology.animal, medicine, Animals, Urea, Pharmacology (medical), Immunodeficiency, Pharmacology, Mucous Membrane, Transmission (medicine), Monkey Diseases, Simian immunodeficiency virus, medicine.disease, Macaca mulatta, Virology, HIV Reverse Transcriptase, Reverse transcriptase, Microbicides for sexually transmitted diseases, Administration, Intravaginal, Infectious Diseases, HIV-1, Vaginal Creams, Foams, and Jellies, Female, Simian Immunodeficiency Virus, Ex vivo |
| الوصف: | The transmission of both cell-free and cell-associated immunodeficiency viruses has been demonstrated directly in multiple animal species and possibly occurs in humans, as suggested by genotyping of the infecting human immunodeficiency virus (HIV) in acutely infected women and in semen from their partners. Therefore, a microbicide may need to block both mechanisms of HIV transmission to achieve maximum efficacy. To date, most of the preclinical evaluation of candidate microbicides has been performed using cell-free HIV. New models of mucosal transmission of cell-associated HIV are needed to evaluate candidate microbicide performance. The MIV-150/zinc acetate/carrageenan (MZC) gel protects Depo-Provera-treated macaques against cell-free simian-human immunodeficiency virus reverse transcriptase (SHIV-RT) infection when applied vaginally up to 8 h before challenge. We recently demonstrated the potent activity of MZC gel against cell-free SHIV-RT in macaque vaginal explants. In the current study, we established a cell-associated SHIV-RT infection model of macaque vaginal tissues and tested the activity of MZC gel in this model. MZC gel protected tissues against cell-associated SHIV-RT infection when present at the time of viral exposure or when applied up to 4 days prior to viral challenge. These data support clinical testing of the MZC gel. Overall, our ex vivo model of cell-associated SHIV-RT infection in macaque vaginal mucosa complements the cell-free infection models, providing tools for prioritization of products that block both modes of HIV transmission. |
| تدمد: | 1098-6596 0066-4804 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::34b64a07874101a9dd404f34cb036955 https://doi.org/10.1128/aac.00073-15 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....34b64a07874101a9dd404f34cb036955 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10986596 00664804 |
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