Production of Polyclonal Antisera that Recognize and Distinguish Between the Extracellular Domains of Neuronal Nicotinic Acetylcholine Receptor Subunits
| العنوان: | Production of Polyclonal Antisera that Recognize and Distinguish Between the Extracellular Domains of Neuronal Nicotinic Acetylcholine Receptor Subunits |
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| المؤلفون: | Maryka Quik, Kelly Dineley-Miller, Jim Patrick, David Char, Shawn Neff |
| المصدر: | Journal of Neurochemistry. 64:332-339 |
| بيانات النشر: | Wiley, 2002. |
| سنة النشر: | 2002 |
| مصطلحات موضوعية: | Protein subunit, Blotting, Western, Molecular Sequence Data, Enzyme-Linked Immunosorbent Assay, Peptide, Receptors, Nicotinic, Transfection, Biochemistry, Chromatography, Affinity, Cell Line, Cellular and Molecular Neuroscience, Affinity chromatography, Extracellular, Animals, Amino Acid Sequence, Acetylcholine receptor, Neurons, chemistry.chemical_classification, Antiserum, biology, Immune Sera, Peptide Fragments, Nicotinic acetylcholine receptor, chemistry, Polyclonal antibodies, biology.protein, Female, Rabbits |
| الوصف: | Ligand-gated ion channels are oligomeric transmembrane proteins that usually contain more than one kind of monomer. The variety of monomers available to participate in oligomer formation and the apparent latitude in acceptable monomer combinations allows considerable diversity. Mechanisms for identifying the monomers comprising specific receptors are needed. We have generated affinity-purified polyclonal antisera that recognize the extracellular domain of nine neuronal nicotinic acetylcholine receptor (nAChR) subunits and distinguish between them. We prepared these antisera by immunizing rabbits with bacterially expressed recombinant protein representing the N-terminal extracellular domain of each neuronal nAChR subunit followed by affinity purification of antibodies against synthetic peptides corresponding to residues 68-81 of the alpha 1 subunit. We demonstrate subunit specificity of each affinity-purified antisera by western blots of the bacterially expressed protein and immunoblot against peptide. We further used these antibodies to demonstrate expression of neuronal nAChR subunits on the surface of transiently transfected simian kidney (COS-7) cells. |
| تدمد: | 1471-4159 0022-3042 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::34e8c2c343af154ddc9dbce462eba9b5 https://doi.org/10.1046/j.1471-4159.1995.64010332.x |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....34e8c2c343af154ddc9dbce462eba9b5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14714159 00223042 |
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