Gold-nanofève surface-enhanced Raman spectroscopy visualizes hypotaurine as a robust anti-oxidant consumed in cancer survival
| العنوان: | Gold-nanofève surface-enhanced Raman spectroscopy visualizes hypotaurine as a robust anti-oxidant consumed in cancer survival |
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| المؤلفون: | Mitsuyo Ohmura, Hideaki Nagashima, Makoto Suematsu, Hiroyuki Watanabe, Yuki Sugiura, Akiko Kubo, Megumi Shiota, Oltea Sampetrean, Takeharu Tani, Yasuaki Kabe, Keiyo Takubo, Takayuki Morikawa, Osamu Nagano, Hideyuki Saya, Takako Hishiki, Shogo Yamazoe, Hiroyuki Takahashi, Takehiro Yamamoto, Kentaro Suina, Daisuke Koike, Mai Itoh, Nobuyoshi Hiraoka, Masayuki Naya |
| المصدر: | Nature Communications, Vol 9, Iss 1, Pp 1-16 (2018) Nature Communications |
| بيانات النشر: | Nature Portfolio, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, Taurine, genetic structures, Science, General Physics and Astronomy, Metal Nanoparticles, Hypotaurine, Mice, SCID, Spectrum Analysis, Raman, General Biochemistry, Genetics and Molecular Biology, Antioxidants, Article, 03 medical and health sciences, chemistry.chemical_compound, Mice, Downregulation and upregulation, Neoplasms, medicine, Animals, Humans, lcsh:Science, Transaminases, Multidisciplinary, biology, CD44, Cancer, General Chemistry, Glutathione, Surface-enhanced Raman spectroscopy, medicine.disease, Mice, Inbred C57BL, 030104 developmental biology, chemistry, Liver, Cancer cell, biology.protein, Biophysics, lcsh:Q, Female, Gold |
| الوصف: | Gold deposition with diagonal angle towards boehmite-based nanostructure creates random arrays of horse-bean-shaped nanostructures named gold-nanofève (GNF). GNF generates many electromagnetic hotspots as surface-enhanced Raman spectroscopy (SERS) excitation sources, and enables large-area visualization of molecular vibration fingerprints of metabolites in human cancer xenografts in livers of immunodeficient mice with sufficient sensitivity and uniformity. Differential screening of GNF-SERS signals in tumours and those in parenchyma demarcated tumour boundaries in liver tissues. Furthermore, GNF-SERS combined with quantum chemical calculation identified cysteine-derived glutathione and hypotaurine (HT) as tumour-dominant and parenchyma-dominant metabolites, respectively. CD44 knockdown in cancer diminished glutathione, but not HT in tumours. Mechanisms whereby tumours sustained HT under CD44-knockdown conditions include upregulation of PHGDH, PSAT1 and PSPH that drove glycolysis-dependent activation of serine/glycine-cleavage systems to provide one-methyl group for HT synthesis. HT was rapidly converted into taurine in cancer cells, suggesting that HT is a robust anti-oxidant for their survival under glutathione-suppressed conditions. Surface-enhanced Raman spectroscopy (SERS) visualizes fingerprints of intermolecular vibrations of many metabolites. Here the authors report a SERS imaging technique that enables the visualization of metabolites distribution and automated extraction of tumour boundaries in frozen tissues. |
| اللغة: | English |
| تدمد: | 2041-1723 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::35948a3e8e227cefaf7d2deca740dd10 https://doaj.org/article/6370f21a7dce45e4b681376a2ad703d3 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....35948a3e8e227cefaf7d2deca740dd10 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20411723 |
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