Optimization of Nano-encapsulation on Neonatal Porcine Islet-like Cell Clusters Using Polymersomes
| العنوان: | Optimization of Nano-encapsulation on Neonatal Porcine Islet-like Cell Clusters Using Polymersomes |
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| المؤلفون: | Jung-Taek Kang, Hyun-Ouk Kim, Sang-Hoon Lee |
| المصدر: | Nanoscale Research Letters Nanoscale Research Letters, Vol 16, Iss 1, Pp 1-11 (2021) |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Polymersomes, Materials science, Cell, Nano-encapsulation, 02 engineering and technology, Extracellular matrix, 03 medical and health sciences, chemistry.chemical_compound, lcsh:TA401-492, medicine, General Materials Science, Bovine serum albumin, Bifunctional, 030304 developmental biology, chemistry.chemical_classification, 0303 health sciences, Lactide, Nano Express, biology, technology, industry, and agriculture, Polymer, 021001 nanoscience & nanotechnology, Condensed Matter Physics, Porcine islets, medicine.anatomical_structure, chemistry, Polymersome, Biophysics, biology.protein, lcsh:Materials of engineering and construction. Mechanics of materials, Amine gas treating, 0210 nano-technology |
| الوصف: | ObjectiveResearches proving methods for nano encapsulation of neonatal porcine islet-like cell clusters (NPCCs) using polymersomes (PSomes) formed using polymers of polyethylene glycol-block-poly lactide (PEG-b-PLA). Herein, our studies present efficient nano encapsulation procedure with minimal damage and loss of NPCCs. MethodsWe used N-hydroxysuccinimide (NHS) on the N-terminal of PSomes to induce binding of amine groups in the extracellular matrix surrounding NPCCs. F-10 culture medium with bovine serum albumin was used in the nano-encapsulation procedure to minimize damage and loss of NPCCs. Finally, we induced crosslinking between bi-functional PSomes (NHS-/NH2-PSomes). ResultsF-10 culture medium containing 0.25% BSA with pH of 7.3 minimized the damage and loss of NPCCs after nano-encapsulation as compared with using basic HBSS buffer (pH 8.0). Also, we induced the efficiency nano encapsulation through conjugation of PSomes using bi-functional PSomes (NHS-/NH2-PSomes). |
| تدمد: | 1556-276X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::35b4a3fef2812abb121462cd476e5615 https://doi.org/10.1186/s11671-021-03512-3 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....35b4a3fef2812abb121462cd476e5615 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 1556276X |
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