Conservation and sex-specific splicing of the transformer gene in the calliphorids Cochliomyia hominivorax, Cochliomyia macellaria and Lucilia sericata
| العنوان: | Conservation and sex-specific splicing of the transformer gene in the calliphorids Cochliomyia hominivorax, Cochliomyia macellaria and Lucilia sericata |
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| المؤلفون: | Fang Li, Esther J. Belikoff, Maxwell J. Scott, Steven P. Vensko |
| المصدر: | PLoS ONE PLoS ONE, Vol 8, Iss 2, p e56303 (2013) |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Male, RNA Splicing, Molecular Sequence Data, lcsh:Medicine, Gene Expression, Genes, Insect, Lucilia, Conserved sequence, Gene Splicing, Molecular Genetics, Exon, Molecular Cell Biology, Morphogenesis, Genetics, Animals, RNA, Messenger, Nucleotide Motifs, lcsh:Science, Biology, Conserved Sequence, Sex Characteristics, Evolutionary Biology, Multidisciplinary, biology, Base Sequence, Evolutionary Developmental Biology, Diptera, lcsh:R, fungi, Intron, Computational Biology, Sex Determination, biology.organism_classification, RNA processing, Lucilia cuprina, RNA splicing, lcsh:Q, Female, Drosophila melanogaster, Cochliomyia hominivorax, Research Article, Developmental Biology |
| الوصف: | Transformer (TRA) promotes female development in several dipteran species including the Australian sheep blowfly Lucilia cuprina, the Mediterranean fruit fly, housefly and Drosophila melanogaster. tra transcripts are sex-specifically spliced such that only the female form encodes full length functional protein. The presence of six predicted TRA/TRA2 binding sites in the sex-specific female intron of the L. cuprina gene suggested that tra splicing is auto-regulated as in medfly and housefly. With the aim of identifying conserved motifs that may play a role in tra sex-specific splicing, here we have isolated and characterized the tra gene from three additional blowfly species, L. sericata, Cochliomyia hominivorax and C. macellaria. The blowfly adult male and female transcripts differ in the choice of splice donor site in the first intron, with males using a site downstream of the site used in females. The tra genes all contain a single TRA/TRA2 site in the male exon and a cluster of four to five sites in the male intron. However, overall the sex-specific intron sequences are poorly conserved in closely related blowflies. The most conserved regions are around the exon/intron junctions, the 3' end of the intron and near the cluster of TRA/TRA2 sites. We propose a model for sex specific regulation of tra splicing that incorporates the conserved features identified in this study. In L. sericata embryos, the male tra transcript was first detected at around the time of cellular blastoderm formation. RNAi experiments showed that tra is required for female development in L. sericata and C. macellaria. The isolation of the tra gene from the New World screwworm fly C. hominivorax, a major livestock pest, will facilitate the development of a "male-only" strain for genetic control programs. |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::35ff9abf3390fd70c680a218c854a4af https://pubmed.ncbi.nlm.nih.gov/23409170 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....35ff9abf3390fd70c680a218c854a4af |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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