Evaluation of serum Nestin and HOTAIR rs12826786 C>T polymorphism as screening tools for breast cancer in Egyptian women
| العنوان: | Evaluation of serum Nestin and HOTAIR rs12826786 C>T polymorphism as screening tools for breast cancer in Egyptian women |
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| المؤلفون: | Sarah Aglan, Heba G. El-Sheredy, Mohamed H Sultan, Eman A El-Bakoury, Omar Elsammak, Ahmed M. Awad, Mohamed Y. Elsammak, Yasser S Ahmed |
| المصدر: | Journal of Medical Biochemistry Journal of Medical Biochemistry, Vol 40, Iss 1, Pp 17-25 (2021) |
| بيانات النشر: | Society of Medical Biochemists of Serbia, Belgrade, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Oncology, medicine.medical_specialty, Clinical Biochemistry, Mammary gland, medicine.disease_cause, lcsh:Biochemistry, 03 medical and health sciences, 0302 clinical medicine, Breast cancer, Cancer stem cell, Internal medicine, Genotype, medicine, lcsh:QD415-436, Original Paper, business.industry, Biochemistry (medical), Myoepithelial cell, HOTAIR, Nestin, medicine.disease, 030104 developmental biology, medicine.anatomical_structure, 030220 oncology & carcinogenesis, business, Carcinogenesis |
| الوصف: | Nestin is a neural stem cell protein that plays an important role in cancer stem cells (CSC) development and proliferation. It has been identified as a marker for newly formed endothelial cells and was shown to be preferentially expressed in basal and myoepithelial cells of the mammary gland.We evaluated serum Nestin and thePatients with breast cancer had a median (Min-Max) of serum Nestin 31.3 (6.7-167.3 pg/mL), while control subjects had a median (Min-Max) of serum Nestin 42.3 (25.7-315.95) pg/mL. The best cut-off value for serum Nestin to differentiate normal subjects and patients with breast cancer was 39.9 pg/mL. This cut-off value had a diagnostic sensitivity of 84.8% and specificity of 65.1%. There was a significant difference in the distribution of different alleles in patients with breast cancer than normal subjects (P=0.039 Exact Fisher test). The breast cancer patients group had 23.9% CC, 52.1% CT, and 23.9% TT genotypes, respectively, while the control group had 46.9% CC, 42.8% CT, and 10.2% TT, respectively.A significantly low serum Nestin below 39.9 pg/mL and a higher percentage of the T/T homozygous variant allele ofNestin je protein neuronskih matičnih ćelija koji ima važnu ulogu u razvoju i proliferaciji matičnih ćelija raka (CSC). Identifikovan je kao marker za novoformirane endotelne ćelije, a pokazalo se da je preferencijalno izražen u bazalnim i mioepitelnim ćelijama mlečne žlezde.Izvršena je evaluacija serumskog Nestina i HOTAIR rs12826786 CT polimorfizma kod zdravih žena u Egiptu, kao i onih sa karcinomom dojke, kao mogućeg skrining alata za identifikaciju pacijenata sa karcinomom dojke. Takođe, testirali smo moguću povezanost između ova dva markera i agresivnosti bolesti.Pacijentkinje sa karcinomom dojke su imale srednju vrednost (Min-Maks) seruma Nestina 31,3 (6,7-167,3 pg/mL), dok su kontrolne ispitanice imale srednju vrednost (Min-Maks) seruma Nestina 42,3 (25,7-315,95 pg/mL). Najbolja granična vrednost Nestina u serumu koja je ukazivala na razliku između zdravih ispitanica i pacijentkinja sa karcinomom dojke bila je 39,9 pg/mL. Ova granična vrednost imala je dijagnostičku osetljivost od 84,8% i specifičnost od 65,1%. Bilo je značajne razlike u distribuciji različitih alela kod pacijentkinja sa karcinomom dojke nego kod zdravih ispitanica (P=0,039 Fišerov test tačne verovatnoće). Grupa bolesnica sa karcinomom dojke imala je 23,9% CC, 52,1% CT i 23,9% TT genotipa, dok je kontrolna grupa imala 46,9% CC, 42,8% CT i 10,2% TT respektivno.Kod egipatskih pacijentkinja koje su imale karcinom dojke otkriven je značajno nizak serumski Nestin, ispod 39,9 pg/mL, i veći procenat alela homozigotne varijante T/T HOTAIR rs12826786 CT. Predlažemo da se prijavljena granična vrednost Nestina u serumu i prisustvo C/T polimorfizma koristi za procenu rizika kod žena za razvoj raka dojke, a može biti i od koristi u skriningu bolesti. Potrebne su obimnije studije u različitim etničkim grupama da bi se naši nalazi potvrdili. |
| اللغة: | English |
| تدمد: | 1452-8266 1452-8258 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::361443c72cb477112da46b742e10e014 http://europepmc.org/articles/PMC7857851 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....361443c72cb477112da46b742e10e014 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14528266 14528258 |
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