Der f 38 Is a Novel TLR4-Binding Allergen Related to Allergy Pathogenesis from Dermatophagoides farinae
| العنوان: | Der f 38 Is a Novel TLR4-Binding Allergen Related to Allergy Pathogenesis from Dermatophagoides farinae |
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| المؤلفون: | Eun-Ju Yang, Minseo Yang, Ji-Sook Lee, Ji-Young Mun, Beom-seok Park, Geunyeong Kim, Yujin Hong, Ayesha Kashif, Hyosun Choi, In-Sik Kim, Soo Jin Lee, Minhwa Hong, Ran-Sook Woo |
| المصدر: | International Journal of Molecular Sciences, Vol 22, Iss 8440, p 8440 (2021) International Journal of Molecular Sciences Volume 22 Issue 16 |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Allergy, QH301-705.5, Caspase 3, Immunoglobulin E, medicine.disease_cause, Catalysis, Inorganic Chemistry, Pathogenesis, Allergen, medicine, TLR4, Physical and Theoretical Chemistry, Biology (General), Receptor, Molecular Biology, house dust mite, QD1-999, Spectroscopy, House dust mite, biology, Chemistry, Organic Chemistry, Der f 38, General Medicine, biology.organism_classification, medicine.disease, allergy, Molecular biology, Computer Science Applications, biology.protein |
| الوصف: | It is difficult to treat allergic diseases including asthma completely because its pathogenesis remains unclear. House dust mite (HDM) is a critical allergen and Toll-like receptor (TLR) 4 is a member of the toll-like receptor family, which plays an important role in allergic diseases. The purpose of this study was to characterize a novel allergen, Der f 38 binding to TLR4, and unveil its role as an inducer of allergy. Der f 38 expression was detected in the body and feces of Dermatophagoides farinae (DF). Electron microscopy revealed that it was located in the granule layer, the epithelium layer, and microvilli of the posterior midgut. The skin prick test showed that 60% of allergic subjects were Der f 38-positive. Der f 38 enhanced surface 203c expression in basophils of Der f 38-positive allergic subjects. By analysis of the model structure of Der p 38, the expected epitope sites are exposed on the exterior side. In animal experiments, Der f 38 triggered an infiltration of inflammatory cells. Intranasal (IN) administration of Der f 38 increased neutrophils in the lung. Intraperitoneal (IP) and IN injections of Der f 38 induced both eosinophils and neutrophils. Increased total IgE level and histopathological features were found in BALB/c mice treated with Der f 38 by IP and IN injections. TLR4 knockout (KO) BALB/c mice exhibited less inflammation and IgE level in the sera compared to wild type (WT) mice. Der f 38 directly binds to TLR4 using biolayer interferometry. Der f 38 suppressed the apoptosis of neutrophils and eosinophils by downregulating proteins in the proapoptotic pathway including caspase 9, caspase 3, and BAX and upregulating proteins in the anti-apoptotic pathway including BCL-2 and MCL-1. These findings might shed light on the pathogenic mechanisms of allergy to HDM. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1661-6596 1422-0067 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::361d46db58d91dee67e7a3d6fa8c4c29 https://www.mdpi.com/1422-0067/22/16/8440 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....361d46db58d91dee67e7a3d6fa8c4c29 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16616596 14220067 |
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