Field evaluation of a Pan-Lassa rapid diagnostic test during the 2018 Nigerian Lassa fever outbreak
| العنوان: | Field evaluation of a Pan-Lassa rapid diagnostic test during the 2018 Nigerian Lassa fever outbreak |
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| المؤلفون: | Ekaete Alice Tobin, Matthew L. Boisen, Meike Pahlmann, Chris Aire, Diana K. S. Nelson, John S. Schieffelin, Adeyemi T. Kayode, Luis M. Branco, Solomon Ehikhametalor, Onikepe A. Folarin, Patience Akhilomen, Jacqueline Agbukor, Danny Asogun, Donatus I Adomeh, Ikponmwosa Odia, Grace Okonofua, John Aiyepada, Katherine J. Siddle, Blessing Osiemi, Augustine Goba, Ephraim Ogbaini-Emovan, Megan L. Heinrich, Kayla G. Barnes, Peter O. Okokhere, John Demby Sandi, Megan M. Rowland, Philomena Eromon, Pardis C. Sabeti, Robert F. Garry, Ekene B. Muoebonam, Samar B. Mehta, Eghosa Uyigue, Christian T. Happi, Michael Airende, Stephan Günther, Sophie Duraffour, Lisa Oestereich, Sylvanus Okogbenin, Omigie Omoregie, Donald S. Grant, Duane J. Bush, George O. Akpede, Mambu Momoh, Wiebke Böhm |
| المصدر: | Scientific Reports, Vol 10, Iss 1, Pp 1-14 (2020) Scientific Reports |
| بيانات النشر: | Nature Publishing Group, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Adult, Male, 0301 basic medicine, Point-of-Care Systems, viruses, Immunology, Nigeria, lcsh:Medicine, Antibodies, Viral, medicine.disease_cause, Sensitivity and Specificity, Microbiology, Article, Virus, Disease Outbreaks, Sierra leone, Young Adult, 03 medical and health sciences, Lassa Fever, 0302 clinical medicine, Case fatality rate, parasitic diseases, Humans, Medicine, 030212 general & internal medicine, Lassa virus, Lassa fever, lcsh:Science, Antigens, Viral, Rapid diagnostic test, Multidisciplinary, biology, Diagnostic Tests, Routine, Sequence Analysis, RNA, business.industry, lcsh:R, Outbreak, Middle Aged, medicine.disease, Virology, 030104 developmental biology, biology.protein, RNA, Viral, Female, lcsh:Q, Antibody, business |
| الوصف: | Lassa virus (LASV) is the causative agent of Lassa fever (LF), an often-fatal hemorrhagic disease. LF is endemic in Nigeria, Sierra Leone and other West African countries. Diagnosis of LASV infection is challenged by the genetic diversity of the virus, which is greatest in Nigeria. The ReLASV Pan-Lassa Antigen Rapid Test (Pan-Lassa RDT) is a point-of-care, in vitro diagnostic test that utilizes a mixture of polyclonal antibodies raised against recombinant nucleoproteins of representative strains from the three most prevalent LASV lineages (II, III and IV). We compared the performance of the Pan-LASV RDT to available quantitative PCR (qPCR) assays during the 2018 LF outbreak in Nigeria. For patients with acute LF (RDT positive, IgG/IgM negative) during initial screening, RDT performance was 83.3% sensitivity and 92.8% specificity when compared to composite results of two qPCR assays. 100% of samples that gave Ct values below 22 on both qPCR assays were positive on the Pan-Lassa RDT. There were significantly elevated case fatality rates and elevated liver transaminase levels in subjects whose samples were RDT positive compared to RDT negative. |
| اللغة: | English |
| تدمد: | 2045-2322 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3650476fbeebe73d67d8b00cc2d89c47 http://link.springer.com/article/10.1038/s41598-020-65736-0 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....3650476fbeebe73d67d8b00cc2d89c47 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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