Species and population specific gene expression in blood transcriptomes of marine turtles
| العنوان: | Species and population specific gene expression in blood transcriptomes of marine turtles |
|---|---|
| المؤلفون: | Lisa M. Komoroske, Jessy R. Hapdei, Margaret M. Lamont, James Benge, Shreya M. Banerjee, Summer Martin, Eleanor J. Sterling, Marc R. Rice, Nadia B. Fernandez, Lauren Kenyon, Jamie Adkins Stoll, Tomoharu Eguchi, Tammy M. Summers, Richard E. Connon, Kathryn McFadden, Scott R. Benson, Heather S. Harris, Jeffrey A. Seminoff, Rebecca L. Lewison, Peter H. Dutton, Camryn D. Allen, Jennifer M. Lynch, Eugenia Naro-Maciel, George H. Balazs, T. Todd Jones |
| المصدر: | BMC genomics, vol 22, iss 1 BMC Genomics, Vol 22, Iss 1, Pp 1-16 (2021) BMC Genomics |
| بيانات النشر: | eScholarship, University of California, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0106 biological sciences, Minimally invasive sampling, Life on Land, Bioinformatics, Lineage (evolution), Foraging, RNA-sequencing, QH426-470, 010603 evolutionary biology, 01 natural sciences, Medical and Health Sciences, Article, law.invention, Transcriptome, 03 medical and health sciences, Species Specificity, Comparative transcriptomics, law, Information and Computing Sciences, Genetics, Animals, Turtle (robot), Life Below Water, 030304 developmental biology, 0303 health sciences, Ortholog, biology, Base Sequence, Human Genome, Conservation physiology, Sea turtle, Biological Sciences, biology.organism_classification, Turtles, Evolutionary biology, Evolutionary ecology, Functional genomics, TP248.13-248.65, Blood sampling, Biotechnology |
| الوصف: | BackgroundTranscriptomic data has demonstrated utility to advance the study of physiological diversity and organisms’ responses to environmental stressors. However, a lack of genomic resources and challenges associated with collecting high-quality RNA can limit its application for many wild populations. Minimally invasive blood sampling combined with de novo transcriptomic approaches has great potential to alleviate these barriers. Here, we advance these goals for marine turtles by generating high quality de novo blood transcriptome assemblies to characterize functional diversity and compare global transcriptional profiles between tissues, species, and foraging aggregations.ResultsWe generated high quality blood transcriptome assemblies for hawksbill (Eretmochelys imbricata),loggerhead (Caretta caretta), green (Chelonia mydas), and leatherback (Dermochelys coriacea) turtles. The functional diversity in assembled blood transcriptomes was comparable to those from more traditionally sampled tissues. A total of 31.3% of orthogroups identified were present in all four species, representing a core set of conserved genes expressed in blood and shared across marine turtle species. We observed strong species-specific expression of these genes, as well as distinct transcriptomic profiles between green turtle foraging aggregations that inhabit areas of greater or lesser anthropogenic disturbance.ConclusionsObtaining global gene expression data through non-lethal, minimally invasive sampling can greatly expand the applications of RNA-sequencing in protected long-lived species such as marine turtles. The distinct differences in gene expression signatures between species and foraging aggregations provide insight into the functional genomics underlying the diversity in this ancient vertebrate lineage. The transcriptomic resources generated here can be used in further studies examining the evolutionary ecology and anthropogenic impacts on marine turtles. |
| وصف الملف: | application/pdf |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3663ad49b12763ecfd6d3ed70cde1405 https://escholarship.org/uc/item/2cb4n5ct |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....3663ad49b12763ecfd6d3ed70cde1405 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |