Immunomodulatory and Antioxidant Activity of Green Grass Jelly Leaf Extract (Cyclea barbata Miers.) In Vitro
| العنوان: | Immunomodulatory and Antioxidant Activity of Green Grass Jelly Leaf Extract (Cyclea barbata Miers.) In Vitro |
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| المؤلفون: | Krisnanda Surya Dharma, Lindia Anggraini, Tri Rini Nuringtyas, Rendi Mahadi, Mustafid Rasyiid, Rahma Nurdiyanti |
| المصدر: | Journal of Tropical Biodiversity and Biotechnology; Vol 3, No 3 (2018): December; 73-79 Journal of Tropical Biodiversity and Biotechnology, Vol 3, Iss 3, Pp 73-79 (2018) |
| بيانات النشر: | Universitas Udayana, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | antioxidant, Antioxidant, DPPH, medicine.medical_treatment, green grass jelly, Ethyl acetate, lcsh:Plant culture, Applied Microbiology and Biotechnology, chemistry.chemical_compound, Cyclea barbata Miers, immunomodulator, medicine, Tannin, lcsh:SB1-1110, lcsh:Agriculture (General), Ecology, Evolution, Behavior and Systematics, chemistry.chemical_classification, Ethanol, Chromatography, Extraction (chemistry), lcsh:S1-972, Agricultural and Biological Sciences (miscellaneous), Terpenoid, Thin-layer chromatography, chemistry, General Health Professions, Biotechnology |
| الوصف: | Green grass jelly ( Cyclea barbata Miers.) is known for its benefit to human health especially in supporting body’s immune system and wellness. This research aimed to determine immunomodulatory and antioxidant activity of green grass jelly leaf extracts in vitro. Old leaves were collected as sample then dried and ground to powder. The extraction was done with sohxletation using three different solvents, chloroform, ethyl acetate, and ethanol. The immunomodulatory activity was evaluated by treating the crude extracts at concentrations of 50, 100 , and 500 m g/mL on macrophage s of rat in vitro. Macrophage cells separated form peritoneal fluid used RPMI m edium. Phagocy tosis activity and phagocy tosis capacity of macrophages were performed in vitro using latex beads that suspended in phosphate buffered saline (PBS). The antioxidant activity was measured by spectrophotometry technique with 1,1-diphen y l-2-picr y lhydraz y l (DPPH) solution . All treatments were done three replicates. Detection of the bioactive groups of the extracts was done by Thin Layer Chromatography (TLC). The results showed that ethyl acetate extract has the highest phagocytosis activity followed by chloroform extract and ethanol extract, respectively. Optimum concentration was reached at 100 m g/mL of ethyl acetate extract. The ethyl acetate extract was also the highest antioxidant activity index 7.7 followed by both extracts of chloroform and ethanol similar index value of 6.25 and 6.3, respectively. The ethyl acetate extract has a high immunomodulatory activity and antioxidant activity which contained phenolics, flavonoids, tannin s, and terpenoids. |
| وصف الملف: | application/pdf |
| تدمد: | 2622-0628 2549-6980 2540-9581 2540-9573 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::36880e6a006ce5db8591b36519d9e506 https://doi.org/10.24843/atbes.2018.v02.i01.p03 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....36880e6a006ce5db8591b36519d9e506 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 26220628 25496980 25409581 25409573 |
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