TLR7 Ligation Inhibits TLR8 Responsiveness in IL-27-Primed Human THP-1 Monocytes and Macrophages
| العنوان: | TLR7 Ligation Inhibits TLR8 Responsiveness in IL-27-Primed Human THP-1 Monocytes and Macrophages |
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| المؤلفون: | Carlene Petes, Sameh Basta, Olena Kourko, Katrina Gee, Natalya Odoardi |
| المصدر: | J Innate Immun Journal of Innate Immunity, Pp 1-14 (2021) |
| بيانات النشر: | S. Karger AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Interleukin-27, Chemokine, THP-1 Cells, medicine.medical_treatment, Monocytes, Proinflammatory cytokine, Immunomodulation, Interferon, medicine, Humans, Immunology and Allergy, RNA, Messenger, Interleukin 27, Internal medicine, Inflammation, Toll-like receptor, biology, Macrophages, virus diseases, interferon, TLR7, RC31-1245, Cell biology, Cytokine, Toll-Like Receptor 7, Toll-Like Receptor 8, biology.protein, toll-like receptor, Medicine, Cytokines, Cytokine secretion, Signal Transduction, Research Article, medicine.drug |
| الوصف: | Regulation of proinflammatory cytokine expression is critical in the face of single-stranded RNA (ssRNA) virus infections. Many viruses, including coronavirus and influenza virus, wreak havoc on the control of cytokine expression, leading to the formation of detrimental cytokine storms. Understanding the regulation and interplay between inflammatory cytokines is critical to the identification of targets involved in controlling the induction of cytokine expression. In this study, we focused on how the antiviral cytokine interleukin-27 (IL-27) regulates signal transduction downstream of Toll-like receptor 7 (TLR7) and TLR8 ligation, which recognize endosomal single-stranded RNA. Given that IL-27 alters bacterial-sensing TLR expression on myeloid cells and can inhibit replication of single-stranded RNA viruses, we investigated whether IL-27 affects expression and function of TLR7 and TLR8. Analysis of IL-27-treated THP-1 monocytic cells and THP-1-derived macrophages revealed changes in mRNA and protein expression of TLR7 and TLR8. Although treatment with IL-27 enhanced TLR7 expression, only TLR8-mediated cytokine secretion was amplified. Furthermore, we demonstrated that imiquimod, a TLR7 agonist, inhibited cytokine and chemokine production induced by a TLR8 agonist, TL8-506. Delineating the immunomodulatory role of IL-27 on TLR7 and TLR8 responses provides insight into how myeloid cell TLR-mediated responses are regulated during virus infection. |
| تدمد: | 1662-8128 1662-811X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3759365f781fd5cb2a624120f2048815 https://doi.org/10.1159/000515738 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....3759365f781fd5cb2a624120f2048815 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16628128 1662811X |
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