Preliminary Characterization of El Tor Vibriophage M4
| العنوان: | Preliminary Characterization of El Tor Vibriophage M4 |
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| المؤلفون: | Sayani Das, Amar N. Ghosh |
| المصدر: | Intervirology. 60:149-155 |
| بيانات النشر: | S. Karger AG, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, viruses, Myoviridae, Genome, Viral, medicine.disease_cause, Genome, El Tor, Bacteriophage, 03 medical and health sciences, chemistry.chemical_compound, Microscopy, Electron, Transmission, Virology, medicine, Bacteriophages, Vibrio cholerae, Microbial Viability, biology, Chemistry, Virus Assembly, Temperature, Virion, Hydrogen-Ion Concentration, biology.organism_classification, 030112 virology, Negative stain, Infectious Diseases, Biochemistry, Capsid, DNA, Viral, DNA |
| الوصف: | Aims: The aim of the present study is the preliminary characterization of an El Tor vibriophage M4 (ATCC 51352-B4). Methods: We studied the growth characteristics and sustainability of this phage under various stresses like temperature, pH, and UV. The phage morphology and phage genome were also examined using electron microscopy. Results: Sustainability studies showed that the phage is more stable in acidic conditions, which is very uncommon among vibriophages. Studies also showed that M4 is a thermostable phage and it is inactivated by temperatures above 60°C but, like other phages, UV has a high inactivating effect on it. Morphological and genomic studies by electron microscopy showed that this phage has a long contractile tail and a big head. The genome is linear and about 120 kb in length. The genome also has a high packaging density as the value of Vm (the volume occupied per Da of biological macromolecule) is low for this phage. The phage-bacterial interaction was studied by negative staining as well as ultrathin sectioning methods. Conclusions: M4 belongs to the Myoviridae family and it is generally thermostable. It is prone to destruction by alkali and UV. It also has a large DNA which is densely packed inside of a big capsid. |
| تدمد: | 1423-0100 0300-5526 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3764c851fb513d00e79a2b360e41c0bb https://doi.org/10.1159/000485835 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....3764c851fb513d00e79a2b360e41c0bb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14230100 03005526 |
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