Additional file 2: Fig. S1. The effect of BMI1 on OS cell proliferation, migration and invasion in vitro. A After OS cells were treated with si-NC or si-BMI1 1#, 2# for 48 h, the proliferative viability of OS cells was detected using CCK-8 assay. Sample size = 6. B Colony formation assays were used to detect the colony forming ability of OS cells after treated with si-NC or si-BMI1 1#, 2# for 48 h. Sample size = 3. C Quantification of B. D After treated with si-NC or si-BMI1 1#, 2# for 48 h, transwell assays were performed to determine the migration of OS cells. Sample size = 3. E Quantification of D. F Invasion assays were performed to detect the invasive ability of OS cells after treated with si-NC or si-BMI1 1#, 2# for 48 h. Sample size = 3. G Quantification of F. *p