High-efficiency chromosomal integrative amplification strategy for overexpressing α-amylase in Bacillus licheniformis
| العنوان: | High-efficiency chromosomal integrative amplification strategy for overexpressing α-amylase in Bacillus licheniformis |
|---|---|
| المؤلفون: | Peili Shen, Dandan Niu, Xuelian Liu, Kangming Tian, Kugen Permaul, Suren Singh, Nokuthula Peace Mchunu, Zhengxiang Wang |
| المصدر: | Journal of Industrial Microbiology and Biotechnology. 49 |
| بيانات النشر: | Oxford University Press (OUP), 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Amylases, Bacillus licheniformis, Bacillus, Bioengineering, alpha-Amylases, Applied Microbiology and Biotechnology, Plasmids, Biotechnology |
| الوصف: | Bacillus licheniformis is a well-known platform strain for production of industrial enzymes. However, the development of genetically stable recombinant B. licheniformis for high-yield enzyme production is still laborious. Here, a pair of plasmids, pUB-MazF and pUB'-EX1, were firstly constructed. pUB-MazF is a thermosensitive, self-replicable plasmid. It was able to efficiently cure from the host cell through induced expression of an endoribonuclease MazF, which is lethal to the host cell. pUB′-EX1 is a nonreplicative and integrative plasmid. Its replication was dependent on the thermosensitive replicase produced by pUB-MazF. Transformation of pUB′-EX1 into the B. licheniformis BL-UBM harboring pUB-MazF resulted in both plasmids coexisting in the host cell. At an elevated temperature, and in the presence of isopropyl-1-thio-β-d-galactopyranoside and kanamycin, curing of the pUB-MazF and multiple-copy integration of pUB′-EX1 occurred, simultaneously. Through this procedure, genetically stable recombinants integrated multiple copies of amyS, from Geobacillus stearothermophilus ATCC 31195 were facilely obtained. The genetic stability of the recombinants was verified by repeated subculturing and shaking flask fermentations. The production of α-amylase by recombinant BLiS-002, harboring five copies of amyS, in a 50-l bioreactor reached 50 753 U/ml after 72 hr fermentation. This strategy therefore has potential for production of other enzymes in B. licheniformis and for genetic modification of other Bacillus species. |
| تدمد: | 1476-5535 1367-5435 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::388aed36a7d98632930fe6777c423c19 https://doi.org/10.1093/jimb/kuac009 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....388aed36a7d98632930fe6777c423c19 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14765535 13675435 |
|---|