Septin-microtubule association requires a MAP-like motif unique to Sept9 isoform 1 embedded into septin octamers
| العنوان: | Septin-microtubule association requires a MAP-like motif unique to Sept9 isoform 1 embedded into septin octamers |
|---|---|
| المؤلفون: | Pascal Verdier-Pinard, Gijsje H. Koenderink, Alex Llewellyn, Maxime Gomes, Keisuke Asano, Danièle Salaün, Taro Tachibana, Jindřiška Leischner Fialová, Yuxiang Liu, Gerard Castro Linares, Mira Kuzmić, Manos Mavrakis, Ali Badache, Francois, Mayssa Belhabib |
| المساهمون: | Centre de Recherche en Cancérologie de Marseille (CRCM), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Aix Marseille Université (AMU), Delft University of Technology (TU Delft), Kavli Institute of Nanosciences [Delft] (KI-NANO), Institut FRESNEL (FRESNEL), Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Aix Marseille Université (AMU), MOSAIC (MOSAIC), Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Aix Marseille Université (AMU), University of Shanghai [Shanghai], Osaka City University (OCU), ANR-17-CE13-0014,SEPTIMORF,Rôle des septines dans la morphogenèse des cellules animales(2017), Aix Marseille Université (AMU)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | Journal of Cell Science Journal of Cell Science, Company of Biologists, In press, ⟨10.1101/2021.04.06.438596⟩ |
| بيانات النشر: | Cold Spring Harbor Laboratory, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Gene isoform, 0303 health sciences, Chemistry, fungi, [SDV.BDD.MOR]Life Sciences [q-bio]/Development Biology/Morphogenesis, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], macromolecular substances, Hereditary neuralgic amyotrophy, Septin, medicine.disease, In vitro, Cell biology, [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics, 03 medical and health sciences, 0302 clinical medicine, Microtubule, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], medicine, Motif (music), biological phenomena, cell phenomena, and immunity, 030217 neurology & neurosurgery, Actin, 030304 developmental biology |
| الوصف: | Septins, a family of GTP-binding proteins assembling into higher order structures, interface with the membrane, actin filaments and microtubules, which positions them as important regulators of cytoarchitecture. Septin 9 (Sept9), which is frequently overexpressed in tumors and mutated in hereditary neuralgic amyotrophy (HNA), mediates the binding of septins to microtubules, but the molecular determinants of this interaction remained uncertain. We demonstrate that a short MAP-like motif unique to Sept9 isoform 1 (Sept9_i1) drives septin octamer-microtubule interaction in cells and in vitro reconstitutions. Septin-microtubule association requires polymerizable septin octamers harboring Sept9_i1. Although outside of the MAP-like motif, HNA mutations abrogates this association, identifying a putative regulatory domain. Removal of this domain from Sept9_i1 sequesters septins on microtubules, promotes microtubule stability and alters actomyosin fiber distribution and tension. Thus, we identify key molecular determinants and potential regulatory roles of septin-microtubule interaction, paving the way to deciphering the mechanisms underlying septin associated pathologies. |
| تدمد: | 0021-9533 1477-9137 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::388e18221a63183826708b01cf24c0a7 https://doi.org/10.1101/2021.04.06.438596 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....388e18221a63183826708b01cf24c0a7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00219533 14779137 |
|---|