NGT1 Is Essential for N-Acetylglucosamine-Mediated Filamentous Growth Inhibition and HXK1 Functions as a Positive Regulator of Filamentous Growth in Candida tropicalis
| العنوان: | NGT1 Is Essential for N-Acetylglucosamine-Mediated Filamentous Growth Inhibition and HXK1 Functions as a Positive Regulator of Filamentous Growth in Candida tropicalis |
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| المؤلفون: | Li Xu, Yunjun Yan, Wang Lei, Xuxia Wang, Qiuyu Zhang, Sheng Yuan, Zhiming Hu, Qinghua Zhou |
| المصدر: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 21, Iss 4036, p 4036 (2020) Volume 21 Issue 11 |
| بيانات النشر: | MDPI, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, HXK1, 030106 microbiology, Virulence, Biology, filamentous growth, Catalysis, Article, Microbiology, Inorganic Chemistry, Candida tropicalis, lcsh:Chemistry, 03 medical and health sciences, chemistry.chemical_compound, N-Acetylglucosamine, NGT1, cell growth, Physical and Theoretical Chemistry, N-acetylglucosamine, Candida albicans, Molecular Biology, lcsh:QH301-705.5, Spectroscopy, Cell growth, Organic Chemistry, General Medicine, Pathogenic fungus, biology.organism_classification, Yeast, Computer Science Applications, 030104 developmental biology, chemistry, lcsh:Biology (General), lcsh:QD1-999, Growth inhibition |
| الوصف: | Candida tropicalis is a pathogenic fungus that can cause opportunistic infections in humans. The ability of Candida species to transition between yeast and filamentous growth forms is essential to their ability to undergo environmental adaptation and to maintain virulence. In other fungal species, such as Candida albicans, N-acetylglucosamine (GlcNAc) can induce filamentous growth, whereas it suppresses such growth in C. tropicalis. In the present study, we found that knocking out the GlcNA-specific transporter gene NGT1 was sufficient to enhance C. tropicalis filamentous growth on Lee&rsquo s plus GlcNAc medium. This suggests that GlcNAc uptake into C. tropicalis cells is essential to the disruption of mycelial growth. As such, we further studied how GlcNAc catabolism-related genes were able to influence C. tropicalis filamentation. We found that HXK1 overexpression drove filamentous growth on Lee&rsquo s media containing glucose and GlcNAc, whereas the deletion of the same gene disrupted this filamentous growth. Interestingly, the deletion of the DAC1 or NAG1 genes impaired C. tropicalis growth on Lee&rsquo s plus GlcNAc plates. Overall, these results indicate that HXK1 can serve as a positive regulator of filamentous growth, with excess GlcNAc-6-PO4 accumulation being toxic to C. tropicalis. These findings may highlight novel therapeutic targets worthy of future investigation. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1422-0067 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::389b05202c055a8a53150e18427edb26 http://europepmc.org/articles/PMC7312872 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....389b05202c055a8a53150e18427edb26 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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