Effect of Dapsone Alone and in Combination With Intracellular Antibiotics Against the Biofilm Form of B. Burgdorferi
| العنوان: | Effect of Dapsone Alone and in Combination With Intracellular Antibiotics Against the Biofilm Form of B. Burgdorferi |
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| المؤلفون: | Phyllis R. Freeman, Krithika Murali, Richard I. Horowitz, Gauri Gaur, Eva Sapi |
| المصدر: | BMC Research Notes BMC Research Notes, Vol 13, Iss 1, Pp 1-7 (2020) |
| بيانات النشر: | Research Square Platform LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Combination therapy, medicine.drug_class, 030106 microbiology, Antibiotics, lcsh:Medicine, Dapsone, General Biochemistry, Genetics and Molecular Biology, Microbiology, 03 medical and health sciences, Lyme disease, Antibiotic resistance, medicine, Humans, Borrelia burgdorferi, lcsh:Science (General), lcsh:QH301-705.5, Doxycycline, Lyme Disease, biology, business.industry, Biofilm, lcsh:R, General Medicine, medicine.disease, biology.organism_classification, bacterial infections and mycoses, Anti-Bacterial Agents, Research Note, 030104 developmental biology, lcsh:Biology (General), Biofilms, business, medicine.drug, lcsh:Q1-390 |
| الوصف: | Objective: Lyme disease is a tick-borne, multisystemic disease caused by Borrelia burgdorferi. Standard treatments for early Lyme disease include short courses of oral antibiotics but relapses often occur after discontinuation of treatment. Several studies have suggested that ongoing symptoms may be due to a highly antibiotic resistant form of B. burgdorferi called biofilms. Our recent clinical study reported the successful use of an intracellular mycobacterium persister drug used in treating leprosy, diaminodiphenyl sulfone (dapsone), in combination therapy for the treatment of Lyme disease. In this in vitro study, we evaluated the effectiveness of dapsone individually and in combination with cefuroxime and/or other antibiotics with intracellular activity including doxycycline, rifampin, and azithromycin against Borrelia biofilm forms utilizing crystal violet biofilm mass, and dimethyl methylene blue glycosaminoglycan assays combined with Live/Dead fluorescent microscopy analyses. Results: Dapsone, alone or in various combinations with doxycycline, rifampin and azithromycin produced a significant reduction in the mass and protective glycosaminoglycan layer and overall viability of B. burgdorferi biofilm forms. This in vitro study strongly suggests that dapsone combination therapy could represent a novel and effective treatment option against the biofilm form of B. burgdorferi. |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::38d7174d5b85625fface46bebc74c5a6 https://doi.org/10.21203/rs.3.rs-40534/v1 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....38d7174d5b85625fface46bebc74c5a6 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |