Ultrafast multiplexed detection of SARS-CoV-2 RNA using a rapid droplet digital PCR system
| العنوان: | Ultrafast multiplexed detection of SARS-CoV-2 RNA using a rapid droplet digital PCR system |
|---|---|
| المؤلفون: | Yigang Tong, Zule Cheng, Hao Yin, Yong Qi, Lin Zhou, Hongju Mao, Jianlong Zhao, Zhenhua Wu, Xiaoyu Jian, Nan Shi |
| المصدر: | Biosensors & Bioelectronics |
| بيانات النشر: | Elsevier B.V., 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | SARS-CoV-2 detection, rapid digital PCR system, Coronavirus disease 2019 (COVID-19), RNase P, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Biomedical Engineering, Biophysics, nucleic testing, 02 engineering and technology, Computational biology, Biosensing Techniques, 01 natural sciences, Multiplexing, Sensitivity and Specificity, Article, Electrochemistry, Reference gene, Humans, Digital polymerase chain reaction, microfluidic technology, Chemistry, SARS-CoV-2, 010401 analytical chemistry, RNA, COVID-19, General Medicine, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Running time, COVID-19 Nucleic Acid Testing, RNA, Viral, 0210 nano-technology, Biotechnology |
| الوصف: | We report the first combination of droplet digital and rapid PCR techniques for efficient, accurate, and quantitative detection of SARS-CoV-2 RNA. The presented rapid digital PCR system simultaneously detects two specific targets (ORF1ab and N genes) and one reference gene (RNase P) with a single PCR thermal cycling period around 7 s and the total running time less than 5 min. A clear positive signal could be identified within 115 s via the rapid digital RT-PCR, suggesting its efficiency for the end-point detection. In addition, benchmark tests with serial diluted reference samples of SARS-CoV-2 RNA reveal the excellent accuracy of our system (R2>0.99). More importantly, the rapid digital PCR system gives consistent and accurate detection of low-concentration reference samples, whereas qPCR yields Ct values with significant variations that could lead to false-negative results. Finally, we apply the rapid digital PCR system to analyze clinical samples with both positive and control cases, where results are consistent with qPCR test outcomes. By providing similar accuracy with qPCR while minimizing the detection time-consuming and the false-negative tendency, the presented rapid digital PCR system represents a promising improvement on the rapid diagnosis of COVID-19. |
| اللغة: | English |
| تدمد: | 1873-4235 0956-5663 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::38f09381506cdeceb5580c582996a57f http://europepmc.org/articles/PMC8093165 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....38f09381506cdeceb5580c582996a57f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18734235 09565663 |
|---|