T Cells in Cryptopatch Aggregates Share TCR γ Variable Region Junctional Sequences with γδ T Cells in the Small Intestinal Epithelium of Mice
| العنوان: | T Cells in Cryptopatch Aggregates Share TCR γ Variable Region Junctional Sequences with γδ T Cells in the Small Intestinal Epithelium of Mice |
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| المؤلفون: | Nicholas Whitley, Hiroko Taniguchi, Joseph Thoits, Hao Yuan Cheng, Victoria Camerini, Kimberly L. Kudla, Joanna Halkias, Bradley S. Podd, Kerstin Goth |
| المصدر: | The Journal of Immunology. 176:6532-6542 |
| بيانات النشر: | The American Association of Immunologists, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Male, CD3 Complex, Sequence analysis, T cell, CD3, Immunology, Mice, Nude, chemical and pharmacologic phenomena, Cell Separation, Thymus Gland, Biology, Mice, Antigen, T-Lymphocyte Subsets, Intestine, Small, medicine, Animals, Immunology and Allergy, Intestinal Mucosa, Receptor, Cell Aggregation, Mice, Knockout, Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor, Lasers, T-cell receptor, Receptors, Antigen, T-Cell, gamma-delta, hemic and immune systems, Exons, Gene rearrangement, Molecular biology, Mice, Inbred C57BL, medicine.anatomical_structure, biology.protein, Intraepithelial lymphocyte, Female, Microdissection |
| الوصف: | The role of cryptopatch aggregates in the development of intestinal intraepithelial lymphocytes (IEL) is a matter of controversy. Therefore, an important question is whether T cells in cryptopatch aggregates are lineally related to IEL. We hypothesized that if γδ+ IEL derive from T cells in cryptopatch aggregates, then a clonal relationship would exist between the two populations. To test this hypothesis, we compared the sequence of rearranged TCR gamma variable region 5 genes in γδ+ IEL and cryptopatch cells. We purified IEL by FACS and cryptopatch cells were isolated from frozen sections of the intestine by laser-assisted microdissection. PCR showed that TCR gamma variable region 5 was rearranged in γδ+ IEL and in CD3+ cryptopatch cells, but not in CD3− cryptopatch cells. DNA sequence analysis showed that the frequency of in-frame junctions in cryptopatch aggregates was at a level consistent with positive selection in both wild-type and athymic nude mice. In addition, the predicted amino acid sequences of V-J junctions present in γδ+ IEL and cryptopatch cells were encoded by identical nucleotide sequences. By contrast, the frequency of in-frame joints was significantly reduced in cryptopatch cells isolated from TCR δ-deficient mice, indicating that the enrichment of in-frame joints in cryptopatch cells must normally depend on expression of surface γδ TCR. Our results are consistent with the hypothesis that a subset of γδ+ IEL are related to T cells in cryptopatch aggregates. The precise role of cryptopatch aggregates in intestinal γδ+ T cell homeostasis still needs to be determined. |
| تدمد: | 1550-6606 0022-1767 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::395d90b822197e7c048845ef2a3f1a7f https://doi.org/10.4049/jimmunol.176.11.6532 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....395d90b822197e7c048845ef2a3f1a7f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15506606 00221767 |
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