Flow cytometric estimation of 'labile iron pool' in human white blood cells reveals a positive association with ageing

التفاصيل البيبلوغرافية
العنوان: Flow cytometric estimation of 'labile iron pool' in human white blood cells reveals a positive association with ageing
المؤلفون: Paschalis-Thomas Doulias, Kostas C. Siamopoulos, Dimitrios Galaris, Panagiotis Kanavaros, Chara Vlachou, Christina Boudouri
المصدر: Free radical research. 42(3)
سنة النشر: 2008
مصطلحات موضوعية: Adult, Male, Aging, Leukocytes/cytology, Oxygen/chemistry, Iron, Single sample, Oxidative phosphorylation, Biology, medicine.disease_cause, Biochemistry, Monocytes, Flow cytometry, chemistry.chemical_compound, Monocytes/metabolism, medicine, Leukocytes, Humans, Scattering, Radiation, Aged, chemistry.chemical_classification, Reactive oxygen species, medicine.diagnostic_test, Age Factors, General Medicine, Middle Aged, Flow Cytometry, Molecular biology, Iron/*chemistry, White (mutation), Calcein, Oxygen, Oxidative Stress, chemistry, Ageing, Immunology, Female, Oxidative stress, Flow Cytometry/*methods
الوصف: A small part of cellular iron, usually called 'labile iron pool' (LIP), is not securely stored and has the potential to catalyse the formation of highly reactive oxygen species. The present work estimated LIP levels in human white cells by using the analytical power of flow cytometry. The method relies essentially on already established principles but has the added value of monitoring LIP in different subpopulations of human blood cells concurrently in a single sample. Examination of 41 apparently healthy individuals revealed a positive correlation between LIP levels and the age of the donors (r=0.656, 0.572 and 0.702 for granulocytes, lymphocytes and monocytes, respectively, p
تدمد: 1071-5762
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::398622d38b541c19b63d78018226fda1
https://pubmed.ncbi.nlm.nih.gov/18344120
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....398622d38b541c19b63d78018226fda1
قاعدة البيانات: OpenAIRE