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Circ_0067680 expedites the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells through miR-4429/CTNNB1/Wnt/β-catenin pathway

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العنوان: Circ_0067680 expedites the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells through miR-4429/CTNNB1/Wnt/β-catenin pathway
المؤلفون: Min Yang, Yuansheng Huang, Su Wan
المصدر: Biology Direct, Vol 16, Iss 1, Pp 1-10 (2021)
Biology Direct
بيانات النشر: BMC, 2021.
سنة النشر: 2021
مصطلحات موضوعية: Wnt/β-catenin signaling pathway, QH301-705.5, Immunology, Biology, General Biochemistry, Genetics and Molecular Biology, Bone Marrow, Osteogenesis, Osteogenic differentiation, Humans, CTNNB1, circ_0067680, Biology (General), Wnt Signaling Pathway, Ecology, Evolution, Behavior and Systematics, beta Catenin, Competing endogenous RNA, Applied Mathematics, Research, Mesenchymal stem cell, Wnt signaling pathway, RNA, Cell Differentiation, Mesenchymal Stem Cells, Cell biology, MicroRNAs, Protein kinase domain, Modeling and Simulation, Catenin, Catenin Beta-1, miR-4429, Signal transduction, General Agricultural and Biological Sciences
الوصف: Background Human bone marrow-derived mesenchymal stem cells (hBMSCs) are the primary source of osteoblasts in vivo. Emerging literatures have unveiled that circular RNAs (circRNAs) are actively drawn in the osteogenic differentiation of mesenchymal stem cells (MSCs). This research mainly illuminated the role of circ_0067680 as well as its regulatory mechanism in osteoblastic differentiation. Methods In this study, RT-qPCR was to measure the expression of circ_0067680. Functional assays were implemented to assess the role of circ_0067680 in osteogenic differentiation. Besides, RNA pull down, RIP and luciferase reporter assays were carried out to investigate the regulatory mechanism of circ_0067680. Results Circ_0067680, which derived from its host gene divergent protein kinase domain 2A (C3orf58), was up-regulated during osteogenic differentiation of hBMSCs. Besides, circ_0067680 deficiency impeded the osteoblastic differentiation of hBMSCs. Moreover, circ_0067680 served as a ceRNA via sequestering miR-4429 to regulate the expression of catenin beta 1 (CTNNB1), thereby activating the Wnt/β-catenin signaling pathway. Conclusion Circ_0067680 accelerated hBMSCs osteogenic differentiation by the miR-4429/CTNNB1/Wnt/β-catenin signaling, which might be used as a potential biomarker for osteoblastic differentiation. Graphic abstract
اللغة: English
تدمد: 1745-6150
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::399ebb97b70f4c21fd05cda8ce21d6e2
https://doaj.org/article/ea97cddae6e44fbd9a0d926b29abe357
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....399ebb97b70f4c21fd05cda8ce21d6e2
قاعدة البيانات: OpenAIRE
الوصف
تدمد:17456150