Differential Regulation of Anthocyanins in Cerasus humilis Fruit Color Revealed by Combined Transcriptome and Metabolome Analysis

التفاصيل البيبلوغرافية
العنوان: Differential Regulation of Anthocyanins in Cerasus humilis Fruit Color Revealed by Combined Transcriptome and Metabolome Analysis
المؤلفون: Shaoyu Lang, Xiaolong Ji, Jing Ren, Di Wang, Xingshun Song, Liwei Zhu
المصدر: Forests, Vol 11, Iss 1065, p 1065 (2020)
Forests
Volume 11
Issue 10
بيانات النشر: MDPI AG, 2020.
سنة النشر: 2020
مصطلحات موضوعية: 0106 biological sciences, anthocyanin biosynthesis, genetic structures, Cerasus humilis, Biology, 01 natural sciences, metabolome and transcriptome analyses, Transcriptome, 03 medical and health sciences, Gene expression, Metabolome, Cultivar, Food science, Gene, 030304 developmental biology, 0303 health sciences, food and beverages, Forestry, Differential regulation, lcsh:QK900-989, fruit color, Anthocyanin biosynthesis, gene expression, lcsh:Plant ecology, 010606 plant biology & botany
الوصف: Coloring is an important appearance quality of fruit. In order to evaluate the relationship between metabolites and fruit color, we analyzed the metabolites and transcriptional profiles of two different Cerasus humilis cultivars: &ldquo
RF&rdquo
(cv. Zhangwu, red fruit) and &ldquo
YF&rdquo
(cv. Nongda No.5, yellow fruit). The results of identification and quantification of metabolites showed that there were significant differences in the contents of 11 metabolites between RF and YF. Transcriptomics was used to analyze the expression patterns of genes related to the anthocyanin biosynthesis pathway, and subsequently, the regulation network of anthocyanin biosynthesis was established to explore their relationship with color formation. QRT-PCR, performed for 12 key genes, showed that the expression profiles of the differentially expressed genes were consistent with the results of the transcriptome data. A co-expression analysis revealed that the late genes were significantly positively correlated with most of the different metabolites. The results of the study provide a new reference for improving the fruit color of Cerasus humilis in the future.
وصف الملف: application/pdf
تدمد: 1999-4907
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3a85d28dc2459588b4c71ffab3e28463
https://doi.org/10.3390/f11101065
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....3a85d28dc2459588b4c71ffab3e28463
قاعدة البيانات: OpenAIRE