Hoechst 33258—poly(dG-dC)·poly(dG-dC) Complexes Of Three Types
| العنوان: | Hoechst 33258—poly(dG-dC)·poly(dG-dC) Complexes Of Three Types |
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| المؤلفون: | Strel'tsov Sa, Alexei L. Zhuze |
| المصدر: | Journal of Biomolecular Structure and Dynamics. 26:99-113 |
| بيانات النشر: | Informa UK Limited, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Models, Molecular, Stereochemistry, Polynucleotides, Linear dichroism, chemistry.chemical_compound, Polydeoxyribonucleotides, Structural Biology, Molecular Biology, Fluorescent Dyes, biology, Circular Dichroism, Spectral properties, Helicase, Biological activity, General Medicine, Ligand (biochemistry), Fluorescence, Poly C, Spectrometry, Fluorescence, Monomer, DNA Topoisomerases, Type I, chemistry, Poly G, Bisbenzimidazole, biology.protein, Nucleic Acid Conformation, Spectrophotometry, Ultraviolet, Dimerization, DNA |
| الوصف: | It was found recently that Hoechst 33258, a dsDNA fluorescent dye used in cytological studies, is an efficient inhibitor of the interaction of TATA-box-binding protein with DNA, DNA topoisomerase I, and DNA helicases. In addition it proved to be a radioprotector. Biological activity of Hoechst 33258 may be associated with dsDNA complexes of not only monomeric, but also dimeric type. In this work, the Hoechst 33258 interaction with poly(dG-dC).poly(dG-dC) was studied using UV-vis and fluorescent spectroscopy, circular and flow-type linear dichroism. It was found that Hoechst 33258 formed with poly(dG-dC).poly(dG-dC) complexes of three types, namely, monomeric, dimeric, and, apparently, tetrameric, and their spectral properties were studied. Complexes of monomeric and dimeric types competed with distamycin A, a minor groove ligand, for binding to poly(dG-dC).poly(dG-dC). We proposed that Hoechst 33258 both monomers and dimers form complexes of the external type with poly(dG-dC).poly(dG-dC) from the side of the minor groove. |
| تدمد: | 1538-0254 0739-1102 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3a9578e21eea481896ce8f89bffffcae https://doi.org/10.1080/07391102.2008.10507228 |
| رقم الأكسشن: | edsair.doi.dedup.....3a9578e21eea481896ce8f89bffffcae |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 15380254 07391102 |
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