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Structural and functional conservation of cis-acting RNA elements in coronavirus 5'-terminal genome regions

التفاصيل البيبلوغرافية
العنوان: Structural and functional conservation of cis-acting RNA elements in coronavirus 5'-terminal genome regions
المؤلفون: Markus Fricke, Nadja Karl, Manja Marz, Kevin Lamkiewicz, Daniel Petersen, John Ziebuhr, Ramakanth Madhugiri, Ulrike C. Wend, Robina Scheuer
المصدر: Virology
بيانات النشر: Elsevier BV, 2018.
سنة النشر: 2018
مصطلحات موضوعية: 0301 basic medicine, Coronavirus phylogeny, Replication, Genome, Viral, Regulatory Sequences, Nucleic Acid, Biology, Virus Replication, medicine.disease_cause, Genome, Article, Cell Line, 03 medical and health sciences, Coronavirus 229E, Human, Virology, medicine, Humans, cis-acting RNA element, Nucleic acid structure, RNA structure, Coronavirus, Genetics, Base Sequence, virus diseases, RNA, biology.organism_classification, Stem-loop, Coronavirus NL63, Human, 030104 developmental biology, Viral replication, Regulatory sequence, Nucleic Acid Conformation, RNA, Viral, Betacoronavirus
الوصف: Structure predictions suggest a partial conservation of RNA structure elements in coronavirus terminal genome regions. Here, we determined the structures of stem-loops (SL) 1 and 2 of two alphacoronaviruses, human coronavirus (HCoV) 229E and NL63, by RNA structure probing and studied the functional relevance of these putative cis-acting elements. HCoV-229E SL1 and SL2 mutants generated by reverse genetics were used to study the effects on viral replication of single-nucleotide substitutions predicted to destabilize the SL1 and SL2 structures. The data provide conclusive evidence for the critical role of SL1 and SL2 in HCoV-229E replication and, in some cases, revealed parallels with previously characterized betacoronavirus SL1 and SL2 elements. Also, we were able to rescue viable HCoV-229E mutants carrying replacements of SL2 with equivalent betacoronavirus structural elements. The data obtained in this study reveal a remarkable degree of structural and functional conservation of 5′-terminal RNA structural elements across coronavirus genus boundaries.
Highlights • Conservation of 5'-terminal SL1 and SL2 elements in alpha- and betacoronaviruses. • HCoV-229E reverse genetics data suggest critical role for SL1/2 in viral replication. • Replacement of SL2 in HCoV-229E with betacoronavirus equivalents is tolerated.
تدمد: 0042-6822
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3abb427839c2a463767634cd999975c3
https://doi.org/10.1016/j.virol.2017.11.025
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....3abb427839c2a463767634cd999975c3
قاعدة البيانات: OpenAIRE
الوصف
تدمد:00426822