The Japanese Society for Regenerative Medicine
| العنوان: | The Japanese Society for Regenerative Medicine |
|---|---|
| المؤلفون: | Shinichiro Kobayashi, Tota Kugiyama, Takanobu Hara, Takashi Hamada, Susumu Eguchi, Masaaki Hidaka, Yasuhiro Maruya, Kyoichiro Maekawa, Mitsuhisa Takatsuki, Yusuke Sakai, Akihiko Soyama, Shinichiro Ono, Tomohiko Adachi, Satomi Okada, Toshiyuki Adachi |
| المصدر: | Regenerative Therapy Regenerative Therapy, Vol 14, Iss, Pp 79-86 (2020) |
| بيانات النشر: | Japanese Society for Regenerative Medicine, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, BAS, biliary anastomotic strictures, Pathology, medicine.medical_specialty, medicine.medical_treatment, Biomedical Engineering, Anastomotic healing, Adipose tissue, Adipose-derived stem cell, MSC, mesenchymal stem cell, Anastomosis, CBD, common bile duct, Biomaterials, 03 medical and health sciences, 0302 clinical medicine, APC, allophycocyanin, FBS, fetal bovine serum, Laparotomy, medicine, lcsh:QH573-671, lcsh:R5-920, business.industry, Bile duct, lcsh:Cytology, BMSC, bone marrow stem cells, medicine.disease, VEGF, vascular endothelial growth factor, FGF, fibroblast growth factor, Transplantation, 030104 developmental biology, medicine.anatomical_structure, ADSC, adipose-derived stem cell, Original Article, HGF, hepatocyte growth factor, Biliary anastomosis, Stem cell, business, Wound healing, lcsh:Medicine (General), Infiltration (medical), 030217 neurology & neurosurgery, Cell sheet, Developmental Biology |
| الوصف: | Introduction: Cell sheets consisting of adipose-derived stem cells (ADSCs) have been reported to be effective for wound healing. We conducted this study to clarify the efficacy of ADSC sheets in wound healing at the duct-to-duct biliary anastomotic site in pigs. Methods: Eleven female pigs (20?25 kg) were divided into two groups: biliary anastomosis with an ADSC sheet (n = 6) or without an ADSC sheet (n = 5). To follow the transplanted ADSCs, PKH26GL-labeled sheets were used in one of the ADSC pigs. Two weeks prior to laparotomy, ADSCs were isolated from the lower abdominal subcutaneous adipose tissue. After three passages, ADSCs were seeded on temperature-responsive culture dishes and collected as cell sheets. ADSC sheets were gently transplanted on the anastomotic site. We evaluated specimens by PKH26GL labeling, macroscopic changes, infiltration of inflammatory cells, and collagen content. Results: Labeled ADSCs remained around the bile duct wall. In the no-ADSC group, more adhesion developed at the hepatic hilum as observed during relaparotomy. Histopathological examination showed that the diameter and cross-sectional area of the bile duct wall were decreased in the ADSC group. In the no-ADSC group, a large number of inflammatory cells and more collagen fibers were identified in the bile duct wall. Conclusions: The present study demonstrated that autologous ADSC sheet transplantation reduced hypertrophic changes in the bile duct wall at the anastomotic site. A long-term follow-up is required to evaluate the efficacy of this mechanism in prevention of biliary anastomotic strictures. Regenerative Therapy, 14, pp.79-86; 2020 |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2352-3204 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ad53e2392c2f5e731369bd7d6f3e5ab http://europepmc.org/articles/PMC6970135 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....3ad53e2392c2f5e731369bd7d6f3e5ab |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23523204 |
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