Revealing RCOR2 as a regulatory component of nuclear speckles
| العنوان: | Revealing RCOR2 as a regulatory component of nuclear speckles |
|---|---|
| المؤلفون: | Duxan Arancibia, Anna Lappala, Fabián Guzmán, Carlos A. Rivera, Jeannie T. Lee, Daniel Verbel-Vergara, María Estela Andrés, Marcela González, Gianluca Merello |
| المصدر: | Epigenetics & Chromatin, Vol 14, Iss 1, Pp 1-15 (2021) Epigenetics & Chromatin |
| بيانات النشر: | Research Square Platform LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Cell type, SC35, CoREST, CoREST2, SRRM2, Membrane-less organelles, QH426-470, Biology, Speckle pattern, Transcription (biology), Nuclear Bodies, Nuclear speckles, Gene expression, Genetics, Humans, Nuclear protein, Molecular Biology, Cell Nucleus, RCOR2, Research, Nuclear Proteins, RNA, Compartmentalization (psychology), SON, Cell biology, RCOR1, SRSF2, SRSF7, Co-Repressor Proteins |
| الوصف: | Background Nuclear processes such as transcription and RNA maturation can be impacted by subnuclear compartmentalization in condensates and nuclear bodies. Here, we characterize the nature of nuclear granules formed by REST corepressor 2 (RCOR2), a nuclear protein essential for pluripotency maintenance and central nervous system development. Results Using biochemical approaches and high-resolution microscopy, we reveal that RCOR2 is localized in nuclear speckles across multiple cell types, including neurons in the brain. RCOR2 forms complexes with nuclear speckle components such as SON, SRSF7, and SRRM2. When cells are exposed to chemical stress, RCOR2 behaves as a core component of the nuclear speckle and is stabilized by RNA. In turn, nuclear speckle morphology appears to depend on RCOR2. Specifically, RCOR2 knockdown results larger nuclear speckles, whereas overexpressing RCOR2 leads to smaller and rounder nuclear speckles. Conclusion Our study suggests that RCOR2 is a regulatory component of the nuclear speckle bodies, setting this co-repressor protein as a factor that controls nuclear speckles behavior. |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3b2986a62b14b7fc322daf2b42f3b6b5 https://doi.org/10.21203/rs.3.rs-978769/v1 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....3b2986a62b14b7fc322daf2b42f3b6b5 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |