Authentication of Meat and Meat Products Using Triacylglycerols Profiling and by DNA Analysis
| العنوان: | Authentication of Meat and Meat Products Using Triacylglycerols Profiling and by DNA Analysis |
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| المؤلفون: | Kamila Zdenkova, Monika Jiru, Eliska Cermakova, Vojtech Hrbek, Katerina Demnerova, Diliara Jilkova, Jana Pulkrabova, Jana Hajslova |
| المصدر: | Foods, Vol 9, Iss 1269, p 1269 (2020) Foods Volume 9 Issue 9 |
| بيانات النشر: | MDPI AG, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Health (social science), Computer science, Plant Science, ambient mass spectrometry, Routine practice, lcsh:Chemical technology, 01 natural sciences, Health Professions (miscellaneous), Microbiology, Article, Ambient mass spectrometry, meat, 0404 agricultural biotechnology, triacylglycerols, Multiplex polymerase chain reaction, lcsh:TP1-1185, Food science, 010401 analytical chemistry, 04 agricultural and veterinary sciences, DNA, 040401 food science, DART ion source, 0104 chemical sciences, PCR, authentication, Food Science |
| الوصف: | Two alternative, complementary analytical strategies were successfully used to identify the most common meat species&mdash beef, pork and chicken&mdash in meat products. The first innovative high-throughput approach was based on triacylglycerols fingerprinting by direct analysis in real time coupled with high-resolution mass spectrometry (DART&ndash HRMS). The second was the classic commonly used DNA analysis based on the use of nuclear or mitochondrial DNA in multiplex polymerase chain reaction (mPCR). The DART&ndash HRMS method represents a rapid, high throughput screening method and was shown to have a good potential for the authentication of meat products. Nevertheless, it should be noted that due to a limited number of samples in this pilot study, we present here a proof of concept. More samples must be analyzed by DART&ndash HRMS to build a robust classification model applicable for reliable authentication. To verify the DART&ndash HRMS results, all samples were analyzed by PCRs. Good compliance in samples classification was documented. In routine practice under these conditions, screening based on DART&ndash HRMS could be used for identification of suspect samples, which could be then examined and validated by accurate PCRs. In this way, saving of both labor and cost could be achieved. In the final phase, commercially available meat products from the Czech market were tested using this new strategy. Canned meats&mdash typical Czech sausages and luncheon meats, all with declared content of beef, pork and chicken meat&mdash were used. Compliance with the label declaration was confirmed and no adulteration was found. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2304-8158 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3b2b296f6c4d340d2ebd73cf6e1682e7 https://www.mdpi.com/2304-8158/9/9/1269 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....3b2b296f6c4d340d2ebd73cf6e1682e7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23048158 |
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